Erythrocyte-mediated toxification of 1,8-dinitropyrene: I. Reduction.

Nitroarenes are environmental contaminants produced during incomplete combustion processes. It has been reported that nitroreduction, the most important pathway of nitroarene toxification, occurs mainly in the liver and intestine. In the present study we have shown that red cells also possess the metabolic competence to reduce nitroarenes. In particular, 1,8-dinitropyrene, the nitroarene chosen as model compound, was reduced to the corresponding mono- and diamino-derivatives, 1-amino-8-nitropyrene and 1,8-diaminopyrene, by human lysate supplemented with cofactors.