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  • Two pathogenic species of Pythium: P. aphanidermatum and P. diclinum from a wheat field.

Two pathogenic species of Pythium: P. aphanidermatum and P. diclinum from a wheat field.

Saudi journal of biological sciences (2010-10-01)
Hashem Al-Sheikh
ABSTRACT

During a survey of pathogenic and non-pathogenic Pythium spp. in different localities in Egypt, several isolates of Pythia were obtained and maintained on corn meal agar. Among these isolates, Pythium aphanidermatum and Pythium diclinum were obtained from rhizosphere of wheat plants grown in Dear Attia village, Minia, Egypt. Identification was made using morphological and molecular analyses. P. aphanidermatum and P. diclinum were able to cause reductions in emergence and adulating in wheat in laboratory scale. P. aphanidermatum appeared to be the most aggressive parasite under agar and pot experimental conditions.

MATERIALS
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Sigma-Aldrich
GenElute PCR Clean-Up Kit, sufficient for 70 purifications