The effect of Ti anodized nano-foveolae structure on preosteoblast growth and osteogenic gene expression.

The effect of Ti anodized nano-foveolae structure on preosteoblast growth and osteogenic gene expression.

Journal of nanoscience and nanotechnology (2014-04-18)

Wei-Qiang Yu, Ling Xu, Fu-Qiang Zhang

ABSTRACT

The TiO2 nanotubes by anodization have been extensively studied for medical implant and orthopedic applications because of enhancing bone development. In the present study, a new nano-foveolae structure verified by SEM and AFM was prepared by simulating the nanotubes exfoliation from anodized Ti. MC3T3-E1 preosteoblasts were used to investigate the effects of new nanoscale surface on cell spreading, proliferation, and osteogenic gene expression. The new nano-foveolae structure supported preosteoblast better spreading, more filopodiaes shown by SEM (4 h), and lower proliferation (72 h) than the smooth Ti. By two weeks, the new nanoscale surface induced higher expression of osteogenic markers alkaline phosphatase (ALP), osteopontin (OPN), osteocalcin (OCN), and collagen I (COL I) with real-time RT-PCR compared to the control group. The results of this study suggest that the new nano-foveolae structure provides a favorable surface for functions of bone cells.

MATERIALS

Product Number

Brand

Product Description

369489

Sigma-Aldrich

Titanium, foil, thickness 2.0 mm, 99.7% trace metals basis

460400

Sigma-Aldrich

Titanium, wire, diam. 0.25 mm, 99.7% trace metals basis

GF05925281

Titanium, microfoil, disks, 10mm, thinness 0.1μm, specific density 42.8μg/cm², permanent mylar 3.5μm support, 99.6+%

GF12929744

Titanium, microfoil, disks, 25mm, thinness 0.25μm, specific density 112.6μg/cm², permanent mylar 3.5μm support, 99.6+%

GF16031843

Titanium, mesh, 100x100mm, nominal aperture 0.19mm, wire diameter 0.23mm, 60x60 wires/inch, open area 20%, twill weave

GF54883581

Titanium, rod, 1000mm, diameter 3.0mm, annealed, 99.6+%

GF76772391

Titanium, rod, 100mm, diameter 10mm, annealed, 99.6+%

GF61322699

Titanium, rod, 100mm, diameter 2mm, annealed, 99.6+%

GF23916618

Titanium, microfoil, disks, 25mm, thinness 0.5μm, specific density 225.4μg/cm², permanent mylar 3.5μm support, 99.6+%

GF13959939

Titanium, microfoil, disks, 10mm, thinness 0.5μm, specific density 225.4μg/cm², permanent mylar 3.5μm support, 99.6+%

GF31127712

Titanium, rod, 10mm, diameter 50mm, 99.99+%

GF34106363

Titanium, rod, 1000mm, diameter 1.5mm, annealed, 99.6+%

GF58567067

Titanium, rod, 100mm, diameter 5mm, annealed, 99.6+%

GF04311638

Titanium, rod, 100mm, diameter 6mm, annealed, 99.6+%

GF48167590

Titanium, tube, 100mm, outside diameter 6.1mm, inside diameter 5.1mm, wall thickness 0.5mm, annealed, 99.6+%

GF17705516

Titanium, rod, 200mm, diameter 3.0mm, annealed, 99.6+%

GF65694426

Titanium, tube, 100mm, outside diameter 1.6mm, inside diameter 1.2mm, wall thickness 0.2mm, hard, 99.6+%

GF64061760

Titanium, rod, 1000mm, diameter 16mm, annealed, 99.6+%

GF52551222

Titanium, microfoil, disks, 10mm, thinness 0.25μm, specific density 112.6μg/cm², permanent mylar 3.5μm support, 99.6+%

GF28411703

Titanium, tube, 100mm, outside diameter 10.3mm, inside diameter 8.7mm, wall thickness 0.8mm, annealed, 99.6+%

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Titanium, tube, 1000mm, outside diameter 4.25mm, inside diameter 3.75mm, wall thickness 0.25mm, annealed, 99.6+%

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GF04909653

Titanium, rod, 50mm, diameter 20mm, as drawn, 99.99+%

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Titanium, rod, 200mm, diameter 9.5mm, annealed, 99.99+%

GF65336700

Titanium, rod, 200mm, diameter 2mm, annealed, 99.6+%

GF87716997

Titanium, rod, 100mm, diameter 16mm, as drawn, 99.99+%

GF06082286

Titanium, microfoil, disks, 10mm, thinness 1.0μm, specific density 429μg/cm², permanent mylar 3.5μm support, 99.6+%

GF03916290

Titanium, rod, 490mm, diameter 2mm, annealed, 99.6+%

GF68670366

Titanium, tube, 1220mm, outside diameter 2.03mm, inside diameter 1.55mm, wall thickness 0.24mm, annealed, 99.6+%