- Highly specific targeting of human leukocytes using gold nanoparticle-based biologically active conjugates.
Highly specific targeting of human leukocytes using gold nanoparticle-based biologically active conjugates.
Journal of biomedical nanotechnology (2014-05-09)
Bernhard F Gibbs, Inna M Yasinska, Luigi Calzolai, Douglas Gilliland, Vadim V Sumbayev
PMID24804546
ABSTRACT
In this study we demonstrate for the first time a new approach for highly specific targeting of viable human leukocytes by functionalised gold nanoconjugates made on the basis of citrate-stabilised gold nanoparticles. This immobilisation involves highly specific recognition of the cells of interest by plasma membrane-associated receptor ligands or functional antibodies attached to gold nanoconjugates. Using this technology we managed to deliver pharmacological inhibitors into the target cells. Also we immobilized fully functional human leukocytes utilising both cell lines and rare primary human cells (basophils). This approach demonstrates possibilities of using nanoconjugates for diagnostics, drug delivery and immune therapy.
MATERIALS
Product Number
Brand
Product Description
Gold, tube, 50mm, outside diameter 8mm, inside diameter 7.8mm, wall thickness 0.1mm, as drawn, 99.95%
Gold, insulated wire, 0.5m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%
Gold, insulated wire, 5m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%
Gold, tube, 200mm, outside diameter 2.0mm, inside diameter 1.7mm, wall thickness 0.15mm, as drawn, 99.95%
Gold, microleaf disks, 10mm, thinness 0.5μm, specific density 966μg/cm2, removable support, 99.99+%
Gold, rod, 25mm, diameter 3.0mm, as drawn, 99.95%
Gold, tube, 100mm, outside diameter 3.0mm, inside diameter 2.8mm, wall thickness 0.10mm, as drawn, 99.95%
Gold, insulated wire, 1m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyimide insulation, 99.99%
Gold, tube, 100mm, outside diameter 10.0mm, inside diameter 9.7mm, wall thickness 0.15mm, as drawn, 99.95%
Gold, insulated wire, 0.5m, conductor diameter 0.025mm, insulation thickness 0.005mm, polyester insulation, 99.99%
Gold, insulated wire, 2m, conductor diameter 0.075mm, insulation thickness 0.012mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Gold, tube, 200mm, outside diameter 1.0mm, inside diameter 0.5mm, wall thickness 0.25mm, as drawn, 99.95%
Gold, insulated wire, 0.5m, conductor diameter 0.125mm, insulation thickness 0.016mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Gold, tube, 100mm, outside diameter 1.0mm, inside diameter 0.5mm, wall thickness 0.25mm, as drawn, 99.95%
Gold, microfoil, disks, 10mm, thinness 0.05μm, specific density 101.3μg/cm2, permanent mylar 3.5μm support, 99.99+%
Gold, rod, 100mm, diameter 2.0mm, as drawn, 99.95%
Gold, rod, 25mm, diameter 4.0mm, as drawn, 99.95%
Gold, microfoil, disks, 10mm, thinness 0.01μm, specific density 20.7μg/cm2, permanent mylar 3.5μm support, 99.99+%
Gold, microfoil, disks, 10mm, thinness 0.1μm, specific density 204.1μg/cm2, permanent mylar 3.5μm support, 99.99+%
Gold, rod, 10mm, diameter 6.0mm, as drawn, 99.95%
Gold, insulated wire, 1m, conductor diameter 0.075mm, insulation thickness 0.012mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Gold, insulated wire, 2m, conductor diameter 0.125mm, insulation thickness 0.014mm, polyester insulation, 99.99%
Gold, insulated wire, 0.5m, conductor diameter 0.075mm, insulation thickness 0.012mm, PTFE (polytetrafluoroethylene) insulation, 99.99%
Gold, tube, 50mm, outside diameter 1.0mm, inside diameter 0.5mm, wall thickness 0.25mm, as drawn, 99.95%
Gold, tube, 100mm, outside diameter 1.60mm, inside diameter 0.6mm, wall thickness 0.5mm, as drawn, 99.95%
Gold, rod, 50mm, diameter 3.0mm, as drawn, 99.95%
Gold, insulated wire, 1m, conductor diameter 0.05mm, insulation thickness 0.007mm, polyester insulation, 99.99%