Role of rhBMP-2 and rhBMP-7 in the metabolism and differentiation of osteoblast-like cells cultured on chemically modified titanium surfaces.

Role of rhBMP-2 and rhBMP-7 in the metabolism and differentiation of osteoblast-like cells cultured on chemically modified titanium surfaces.

The Journal of oral implantology (2014-12-17)

Fabiano Ribeiro Cirano, Adriane Yaeko Togashi, Márcia Martins Marques, Francisco Emílio Pustiglioni, Luiz Antônio Pugliesi Alves Lima

PMID25506660

ABSTRACT

This study analyzed the role of recombinant human bone morphogenetic protein 2 (rhBMP-2) and recombinant human bone morphogenetic protein 7 (rhBMP-7) in the adhesion and differentiation of rat osteoblast-like (osteo-1) cells cultured on chemically modified titanium surfaces. Osteo-1 cells were cultured on chemically modified (modified sandblasted and acid-etched) titanium surfaces in 3 different types of medium: control, medium supplemented with 20 ng/mL rhBMP-2, and medium supplemented with 20 ng/mL rhBMP-7. The following parameters were evaluated: cell adhesion after 24 hours; total protein content; collagen content and alkaline phosphatase (AP) activity after 7, 14, and 21 days; and calcified nodule formation after 21 days. The addition of rhBMP-2 or rhBMP-7 did not influence cell adhesion (P = .1175). Cell differentiation was influenced by rhBMP-2, as demonstrated by a significant increase in collagen content after 7 days of culture (P < .0001) and a significant decrease in AP activity after 21 days (P < .0001). The addition of rhBMP-7 only influenced AP activity, and a significant increase was observed after 21 days (P < .0001). Within the limitations of the study, we conclude that the presence of rhBMP-2 or rhBMP-7 did not influence cell adhesion to chemically modified titanium surfaces but provided an additional stimulus during the differentiation of rat osteo-1 cells cultured on this type of surface.

MATERIALS

Product Number

Brand

Product Description

C3867

Sigma-Aldrich

Collagen, Type I solution from rat tail, BioReagent, suitable for cell culture, sterile-filtered

369489

Sigma-Aldrich

Titanium, foil, thickness 2.0 mm, 99.7% trace metals basis

460400

Sigma-Aldrich

Titanium, wire, diam. 0.25 mm, 99.7% trace metals basis

P8361

Sigma-Aldrich

Phosphatase, Alkaline bovine, recombinant, expressed in Pichia pastoris, ≥4000 units/mg protein

C0543

Sigma-Aldrich

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane, Type IV (Miller), lyophilized powder, BioReagent, suitable for cell culture

C3657

Sigma-Aldrich

Collagen from human placenta, Bornstein and Traub Type V (Sigma Type IX), powder

C4407

Sigma-Aldrich

Collagen from human placenta, Bornstein and Traub Type III (Sigma Type X), powder

C7661

Sigma-Aldrich

Collagen from rat tail, Bornstein and Traub Type I, powder, BioReagent, suitable for cell culture

GF24976525

Titanium, rod, 200mm, diameter 25mm, annealed, 99.6+%

GF32801251

Titanium, tube, 1000mm, outside diameter 1.05mm, inside diameter 0.75mm, wall thickness 0.15mm, annealed, 99.6+%

GF88742054

Titanium, tube, 200mm, outside diameter 4.25mm, inside diameter 3.75mm, wall thickness 0.25mm, annealed, 99.6+%

GF91672139

Titanium, wire reel, 20m, diameter 0.05mm, hard, 99.8%

GF56868766

Titanium, wire reel, 25m, diameter 1.0mm, annealed, 99.6+%

GF00761217

Titanium, wire reel, 200m, diameter 0.25mm, as drawn, 99.6+%

GF96391402

Titanium, wire reel, 25m, diameter 0.20mm, annealed, 99.6+%

GF36425707

Titanium, wire reel, 200m, diameter 0.20mm, annealed, 99.6+%

GF31085760

Titanium, wire reel, 5m, diameter 0.25mm, as drawn, 99.99+%

GF43519945

Titanium, wire reel, 2m, diameter 1.0mm, as drawn, 99.99+%

GF34996685

Titanium, wire reel, 200m, diameter 0.125mm, annealed, 99.6+%

GF46592949

Titanium, wire, straight, 1000mm, diameter 1.0mm, as drawn, 99.6+%

GF43403431

Titanium, wire reel, 500m, diameter 0.20mm, annealed, 99.6+%

GF14377631

Titanium, wire reel, 25m, diameter 0.25mm, annealed, 99.6+%

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Titanium, wire reel, 5m, diameter 0.20mm, annealed, 99.6+%

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Titanium, wire reel, 5m, diameter 1.0mm, as drawn, 99.6+%

GF26602321

Titanium, tube, 500mm, outside diameter 25.4mm, inside diameter 23.62mm, wall thickness 0.89mm, annealed, 99.6+%

GF06813525

Titanium, wire reel, 1m, diameter 0.5mm, as drawn, 99.6+%

GF29584187

Titanium, tube, 500mm, outside diameter 2.03mm, inside diameter 1.55mm, wall thickness 0.24mm, annealed, 99.6+%

GF16213553

Titanium, wire reel, 10m, diameter 0.125mm, annealed, 99.6+%

GF16602768

Titanium, wire reel, 1m, diameter 0.5mm, as drawn, 99.99+%

GF72193114

Titanium, tube, 500mm, outside diameter 2.03mm, inside diameter 1.63mm, wall thickness 0.2mm, annealed, 99.6+%