- Engineering cell surfaces for orthogonal selectability.
Engineering cell surfaces for orthogonal selectability.
Angewandte Chemie (International ed. in English) (2012-12-15)
Yingjie Peng, Dae Hee Kim, Teresa M Jones, Diana I Ruiz, Richard A Lerner
PMID23239476
ABSTRACT
Vectors have been constructed that express the chitin-binding domain (ChBD) on eukaryotic cell surfaces. The ChBD is linked to enhanced green fluorescent protein (EGFP) through a protein that spans the plasma membrane. This binding functionality does not have a counterpart in eukaryotes, thereby endowing the modified cell surface with a property that is orthogonal to animal cells.
MATERIALS
Product Number
Brand
Product Description
Roche
X-tremeGENE™ 9 DNA Transfection Reagent, Polymer reagent for transfecting common cell lines