Skip to Content
Merck
CN
  • Effects of physical restraint and electrical stunning on plasma corticosterone, postmortem metabolism, and quality of broiler breast muscle.

Effects of physical restraint and electrical stunning on plasma corticosterone, postmortem metabolism, and quality of broiler breast muscle.

Journal of animal science (2014-11-05)
J C Huang, M Huang, P Wang, L Zhao, X L Xu, G H Zhou, J X Sun
ABSTRACT

The objective of this study was to determine the effects of physical restraint and electrical stunning on plasma corticosterone, postmortem metabolism, and quality of broiler breast muscle. Before slaughter, a total of 160 Arbor Acres broilers were randomly categorized into 2 replicate pens (80 broilers per pen) and every pen was randomly divided into 4 groups (free struggle, physical restraint, free struggle and electrical stunning, and physical restraint and electrical stunning; n=20 per group). Glucose, lactate, and corticosterone were determined on blood plasma samples. Pectoralis major were removed after evisceration and used for determination of meat quality, energy metabolism, and calpain activity. In this study, reducing free struggle by physical restraint combined with electrical stunning improved (P<0.05) meat water holding capacity. Free struggle preslaughter and during bleeding increased (P<0.05) breast muscle redness, energy metabolism, and autolysis of μ/m-calpain and decreased (P<0.05) meat shear values. Physical restraint and electrical stunning decreased (P<0.05) plasma corticosterone level.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Lactic acid, 88%, FCC
Sigma-Aldrich
Corticosterone, ≥98.5% (HPLC)
Supelco
Corticosterone, VETRANAL®, analytical standard
Sigma-Aldrich
Sodium iodoacetate, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
Sodium iodoacetate, ≥98%, BioUltra
Sigma-Aldrich
Lactic acid, meets USP testing specifications
Sigma-Aldrich
Sodium iodoacetate, ≥98%
Sigma-Aldrich
Corticosterone, ≥92%
Supelco
Lactic acid, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Lactic acid, natural, ≥85%
Supelco
Perchloric acid, 0.01 M HClO4 in water (0.01N), eluent for IC
Sigma-Aldrich
DL-Lactic acid, 85 % (w/w), syrup
Sigma-Aldrich
DL-Lactic acid, ~90% (T)
Sigma-Aldrich
Lactic acid solution, ACS reagent, ≥85%
Sigma-Aldrich
Perchloric acid, ACS reagent, 60%
Sigma-Aldrich
Perchloric acid, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., 70.0-72.0%
USP
Lactic acid, United States Pharmacopeia (USP) Reference Standard