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  • Identification of bacteria pathogenic to or associated with onion (Allium cepa) based on sequence differences in a portion of the conserved gyrase B gene.

Identification of bacteria pathogenic to or associated with onion (Allium cepa) based on sequence differences in a portion of the conserved gyrase B gene.

Journal of microbiological methods (2014-06-14)
Jean M Bonasera, Jo Ann E Asselin, Steven V Beer
ABSTRACT

We have developed a method for the identification of Gram-negative bacteria, particularly members of the Enterobacteriaceae, based on sequence variation in a portion of the gyrB gene. Thus, we identified, in most cases to species level, over 1000 isolates from onion bulbs and leaves and soil in which onions were grown.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Xylene Cyanol FF, Dye content ≥75 %
Sigma-Aldrich
Xylene Cyanol FF, BioReagent, Molecular Biology