Merck
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  • Over-expression of nerve growth factor-β in human cholangiocarcinoma QBC939 cells promote tumor progression.

Over-expression of nerve growth factor-β in human cholangiocarcinoma QBC939 cells promote tumor progression.

PloS one (2013-05-03)
Xiu-Jing Yue, Lei-Bo Xu, Man-Sheng Zhu, Rui Zhang, Chao Liu
ABSTRACT

It has been shown that nerve growth factor-β (NGF-β) promoted the initiation and progression of many tumors, and we have previously demonstrated that the expression of NGF-β was associated with tumor stage, nerve infiltration and lymph node metastasis in human hilar cholangiocarcinoma. However, whether NGF-β promotes tumor progression in human cholangiocarcinoma requires further investigation. Therefore, we aimed to determine the effects of NGF-β on the progression of human cholangiocarcinoma. Human cholangiocarcinoma QBC939 stable cell lines with over-expressed or silenced NGF-β genes were generated with pEGFP-N1-NGF-β and pGPU6/GFP/Neo-NGF-β-shRNA recombinant plasmids. Cell proliferation assay, colony formation assay, cell cycle analysis, apoptosis assay and tumorigenicity assay were performed to evaluate the role of NGF-β in the progression of human cholangiocarcinoma. In addition, human lymphatic endothelial cells were co-cultured with QBC939 culture supernatants, and the cell proliferation and migration abilities of the lymphatic endothelial cells were evaluated. Forced expression of NGF-β in QBC939 cell lines promoted proliferation, colony formation and tumorigenicity in these cells and inhibited the apoptosis. However, down-regulation of NGF-β inhibited proliferation, colony formation and tumorigenicity, and increased the apoptotic rate of QBC939 cells. In addition, the NGF-β gain-of-function induced a high expression of vascular endothelial growth factor C and enhanced the proliferation and migration of lymphatic endothelial cells, while NGF-β loss-of-function showed opposite effects. We concluded that NGF-β promoted tumor progression in human cholangiocarcinoma QBC939 cells. Our results provided a new concept to understand the role of NGF-β in cholangiocarcinoma progression, and might provide important information for the development of new targeted therapies in human cholangiocarcinoma.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
NGF-β from rat, recombinant, expressed in Sf21 cells, lyophilized powder, suitable for cell culture
Sigma-Aldrich
Rat β-NGF ELISA Kit, for serum, plasma and cell culture supernatant
Sigma-Aldrich
Human β-NGF ELISA Kit, for serum, plasma, cell culture supernatant and urine