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  • Involvement of PI3K, MAPK ERK1/2 and p38 in Functional Stimulation of Mesenchymal Progenitor Cells by Alkaloid Songorine.

Involvement of PI3K, MAPK ERK1/2 and p38 in Functional Stimulation of Mesenchymal Progenitor Cells by Alkaloid Songorine.

Bulletin of experimental biology and medicine (2015-06-03)
G N Zyuz'kov, V V Zhdanov, L A Miroshnichenko, E V Udut, A V Chaikovskii, E V Simanina, M G Danilets, M Yu Minakova, V V Udut, T G Tolstikova, E E Shults, L A Stavrova, Ya V Burmina, A M Dygai
ABSTRACT

We studied the role of intracellular signaling molecules PI3K, МАРK ERK1/2, and р38 in stimulation of realization of the growth potential of mesenchymal progenitor cells by alkaloid songorine in vitro. Inhibitors of PI3K, ERK1/2 and р38 canceled the increase in proliferative activity of progenitor cells, the blockers of ERK1/2 and р38 reduced the intensity of progenitor cell differentiation.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Hydroxyurea, 98%, powder
SAFC
L-Glutamine
Sigma-Aldrich
L-Glutamine, ReagentPlus®, ≥99% (HPLC)
Sigma-Aldrich
L-Glutamine, γ-irradiated, BioXtra, suitable for cell culture
Sigma-Aldrich
L-Glutamine, BioUltra, ≥99.5% (NT)
Sigma-Aldrich
L-Glutamine
Sigma-Aldrich
L-Glutamine, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source
Sigma-Aldrich
L-Glutamine, Vetec, reagent grade, ≥99%
Sigma-Aldrich
Hydroxyurea, Vetec, reagent grade, ≥98%