Skip to Content

CN

Ivermectin Pharmacokinetics, Metabolism, and Tissue/Egg Residue Profiles in Laying Hens.

Ivermectin Pharmacokinetics, Metabolism, and Tissue/Egg Residue Profiles in Laying Hens.

Journal of agricultural and food chemistry (2015-11-11)

Laura Moreno, Paula Dominguez, Cristina Farias, Lucila Canton, Guillermo Virkel, Laura Maté, Laura Ceballos, Carlos Lanusse, Luis Alvarez

ABSTRACT

The goals were to determine the ivermectin (IVM) plasma pharmacokinetics, tissue and egg residue profiles, and in vitro metabolism in laying hens. Experiments conducted were (1) 8 hens were intravenously treated with IVM and blood samples taken; (2) 88 hens were treated with IVM administered daily in water (5 days) (40 were kept and their daily eggs collected; 48 were sacrificed in groups (n = 8) at different times and tissue samples taken and analyzed); (3) IVM biotransformation was studied in liver microsomes. Pharmacokinetic parameters were AUC = 85.1 ng·day/mL, Vdss = 4.43 L/kg, and T1/2el = 1.73 days. Low IVM tissue residues were quantified with the highest measured in liver and skin+fat. IVM residues were not found in egg white, but significant amounts were quantified in yolk. Residues measured in eggs were greater than some MRL values, suggesting that a withdrawal period would be necessary for eggs after IVM use in laying hens.

MATERIALS

Product Number

Brand

Product Description

Sigma-Aldrich

Methanol, purification grade, 99.8%

Sigma-Aldrich

Methanol, anhydrous, 99.8%

Sigma-Aldrich

Methanol, suitable for NMR (reference standard)

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Trifluoroacetic anhydride, ReagentPlus^®, ≥99%

Sigma-Aldrich

Methanol, ACS spectrophotometric grade, ≥99.9%

Sigma-Aldrich

Methanol, Laboratory Reagent, ≥99.6%

Sigma-Aldrich

Methanol, puriss., meets analytical specification of Ph Eur, ≥99.7% (GC)

Sigma-Aldrich

Methanol, BioReagent, ≥99.93%

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Methanol, ACS reagent, ≥99.8%

Sigma-Aldrich

Methanol, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)

Sigma-Aldrich

Methanol solution, contains 0.50 % (v/v) triethylamine

Sigma-Aldrich

Methanol solution, suitable for NMR (reference standard), 4% in methanol-d₄ (99.8 atom % D), NMR tube size 3 mm × 8 in.

Sigma-Aldrich

Acetonitrile, Preparateur, ≥99.9% (GC), One-time steel-plastic (SP) drum

Sigma-Aldrich

Potassium phosphate dibasic solution, 1.0 M

Sigma-Aldrich

Potassium phosphate dibasic, meets USP testing specifications

Sigma-Aldrich

Potassium phosphate dibasic, anhydrous, suitable for luminescence, Molecular Biology, BioUltra, ≥99.0% (T)

Sigma-Aldrich

Potassium phosphate dibasic, 99.95% trace metals basis

Sigma-Aldrich

Acetonitrile, biotech. grade, ≥99.93%

Sigma-Aldrich

Potassium phosphate dibasic, reagent grade, ≥98.0%

Sigma-Aldrich

Potassium phosphate dibasic, Vetec^™, reagent grade, 98%

Sigma-Aldrich

Potassium phosphate dibasic, puriss. p.a., ACS reagent, anhydrous, ≥99.0% (T)

Sigma-Aldrich

Acetonitrile, electronic grade, 99.999% trace metals basis

Sigma-Aldrich

Acetonitrile, anhydrous, 99.8%

Sigma-Aldrich

Acetonitrile, ReagentPlus^®, 99%

Sigma-Aldrich

Potassium phosphate dibasic, ACS reagent, ≥98%

Sigma-Aldrich

Acetonitrile, ≥99.5% (GC)

Sigma-Aldrich

Acetonitrile, ACS reagent, ≥99.5%

Sigma-Aldrich

Acetonitrile, suitable for DNA synthesis, ≥99.9% (GC)