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  • STEP activation by Gαq coupled GPCRs opposes Src regulation of NMDA receptors containing the GluN2A subunit.

STEP activation by Gαq coupled GPCRs opposes Src regulation of NMDA receptors containing the GluN2A subunit.

Scientific reports (2016-11-20)
Meng Tian, Jian Xu, Gang Lei, Paul J Lombroso, Michael F Jackson, John F MacDonald
ABSTRACT

N-methyl-D-aspartate receptors (NMDARs) are necessary for the induction of synaptic plasticity and for the consolidation of learning and memory. NMDAR function is tightly regulated by functionally opposed families of kinases and phosphatases. Herein we show that the striatal-enriched protein tyrosine phosphatase (STEP) is recruited by Gα

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Sodium orthovanadate, ≥90% (titration)
Sigma-Aldrich
Carbamoylcholine chloride, ≥98% (titration), crystalline
Sigma-Aldrich
PP2, ≥98% (HPLC)
Sigma-Aldrich
N-Methyl-D-aspartic acid, ≥98% (TLC), solid