- Development of the Larval Amphibian Growth and Development Assay: Effects of benzophenone-2 exposure in Xenopus laevis from embryo to juvenile.
Development of the Larval Amphibian Growth and Development Assay: Effects of benzophenone-2 exposure in Xenopus laevis from embryo to juvenile.
Journal of applied toxicology : JAT (2016-06-01)
Jonathan T Haselman, Maki Sakurai, Naoko Watanabe, Yasushi Goto, Yuta Onishi, Yuki Ito, Yu Onoda, Patricia A Kosian, Joseph J Korte, Rodney D Johnson, Taisen Iguchi, Sigmund J Degitz
PMID27241388
ABSTRACT
The Larval Amphibian Growth and Development Assay (LAGDA) is a globally harmonized chemical testing guideline developed by the U.S. Environmental Protection Agency in collaboration with Japan's Ministry of Environment to support risk assessment. The assay is employed as a higher tiered approach to evaluate effects of chronic chemical exposure throughout multiple life stages in a model amphibian species, Xenopus laevis. To evaluate the utility of the initial LAGDA design, the assay was performed using a mixed mode of action endocrine disrupting chemical, benzophenone-2 (BP-2). X. laevis embryos were exposed in flow-through conditions to 0, 1.5, 3.0 or 6.0 mg l
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