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  • Fluorescently labelled multiplex lateral flow immunoassay based on cadmium-free quantum dots.

Fluorescently labelled multiplex lateral flow immunoassay based on cadmium-free quantum dots.

Methods (San Diego, Calif.) (2017-01-28)
Natalia V Beloglazova, Aleksander M Sobolev, Mickael D Tessier, Zeger Hens, Irina Yu Goryacheva, Sarah De Saeger
ABSTRACT

A sensitive tool for simultaneous qualitative detection of two mycotoxins based on use of non-cadmium quantum dots (QDs) is presented for the first time. QDs have proven themselves as promising fluorescent labels for biolabeling and chemical analysis. With an increasing global tendency to regulate and limit the use of hazardous elements, indium phosphide (InP) QDs are highlighted as environmentally-friendly alternatives to the highly efficient and well-studied, but potentially toxic Cd- and Pb-based QDs. Here, we developed water-soluble InP QDs-based fluorescent nanostructures. They consisted of core/shell InP/ZnS QDs enrobed in a silica shell that allowed the water solubility (QD@SiO

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Trioctylphosphine, 97%
Sigma-Aldrich
N-Hydroxysulfosuccinimide sodium salt, ≥98% (HPLC)
Sigma-Aldrich
Tetraethylene glycol monododecyl ether, BioXtra, ≥98.0% (GC)