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  • Mechanisms of toxic action of silver nanoparticles in the protozoan Tetrahymena thermophila: From gene expression to phenotypic events.

Mechanisms of toxic action of silver nanoparticles in the protozoan Tetrahymena thermophila: From gene expression to phenotypic events.

Environmental pollution (Barking, Essex : 1987) (2017-03-21)
Katre Juganson, Monika Mortimer, Angela Ivask, Sandra Pucciarelli, Cristina Miceli, Kaja Orupõld, Anne Kahru
ABSTRACT

Silver nanoparticles (AgNPs) are highly toxic to aquatic organisms, however, there is no consensus whether the toxicity is caused solely by released Ag-ions or also by reactive oxygen species (ROS). Here, the effects of protein-coated AgNPs (14.6 nm, Collargol) were studied on viability, oxidative stress and gene expression levels in wild type strains (CU427 and CU428) of ciliate Tetrahymena thermophila. Viability-based 24 h EC

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Sigma-Aldrich
DCFHDA, BioReagent, suitable for fluorescence, ≥95% (HPLC)