- Headspace SPME-GC-MS metabolomics analysis of urinary volatile organic compounds (VOCs).
Headspace SPME-GC-MS metabolomics analysis of urinary volatile organic compounds (VOCs).
Methods in molecular biology (Clifton, N.J.) (2014-10-02)
Shucha Zhang, Daniel Raftery
PMID25270935
ABSTRACT
In this chapter, we describe the procedure for metabolic profiling of hundreds of urinary volatile organic compounds (VOCs) using headspace solid-phase microextraction (SPME) coupled to gas chromatography-mass spectrometry (GC-MS). SPME is a solvent-free technology that is reproducible, fast, cost effective, and versatile in extracting small molecular weight organic compounds (metabolites) from biofluids using a fiber that is coated with an extracting phase. After extraction, the SPME fiber can be directly injected to the GC-MS, where the extracted metabolites will desorb thermally from the fiber, elute along a GC column, and finally enter into the MS for detection. The analysis of urine samples is presented using this approach.
MATERIALS
Product Number
Brand
Product Description
Supelco
SPME Fiber Assembly, 60 μm PEG, Metal Alloy (1 cm), needle size 23 ga, Manual Holder, pk of 3, purple hub
Supelco
SPME Fiber Assembly, 60 μm PEG, Metal Alloy (1 cm), needle size 23 ga, Autosampler, pk of 3, purple hub
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, Metal Alloy (1 cm), needle size 23 ga, Autosampler, pk of 1, pink hub
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, pink hub
Supelco
SPME Fiber Assembly, 7 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, green hub
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, blue hub
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, blue hub
Supelco
SPME Fiber Assembly, 100 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, red hub
Supelco
SPME Fiber Assembly, 85 μm Polyacrylate, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, white hub
Supelco
SPME Fiber Assembly, 100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, red hub
Supelco
SPME Fiber Assembly, 100 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, red hub
Supelco
SPME Fiber Assembly, 85 μm Polyacrylate, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, white hub
Supelco
SPME Fiber Assembly, 30 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, yellow hub
Supelco
SPME Fiber Assembly, 30 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, yellow hub
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, blue hub
Supelco
SPME Fiber Assembly, 7 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, green hub
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, pink hub
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, blue hub
Supelco
SPME Fiber Assembly, 65 μm PDMS/DVB, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, pink hub
Supelco
SPME Fiber Assembly, 85 μm Polyacrylate, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, white hub
Supelco
SPME Fiber Assembly, 7 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, green hub
Supelco
SPME Fiber Assembly, 100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, red hub
Supelco
SPME Fiber Assembly, 100 μm PDMS, Metal Alloy (1 cm), needle size 23 ga, Autosampler, pk of 1, red hub
Supelco
SPME Overcoated Fiber Assembly, 65 μm PDMS/DVB, StableFlex (1 cm), needle size 23 ga, Autosampler, pk of 3, notched pink hub
Supelco
SPME Fiber Assembly Kit, Assortment #2, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3