Critical aspects for the reliable headspace analysis of plants cultivated in vitro.

Critical aspects for the reliable headspace analysis of plants cultivated in vitro.

Phytochemical analysis : PCA (2001-11-14)

K Maes, J Vercammen, H Pham-Tuan, P Sandra, P C Debergh

ABSTRACT

Various factors controlling the recoveries of volatile organic compounds in vitro headspace analysis of tomato plants (Lycopersicon esculentum Mill. 'Moneymaker'), sampled using solid phase micro-extraction, were evaluated and optimised. The variations in composition of the headspaces were determined as a function of time, and following in vitro wounding of the plant.

MATERIALS

Product Number

Brand

Product Description

57300-U

Supelco

SPME Fiber Assembly, 100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, red hub

504823

Supelco

SPME Portable Field Sampler, coating PDMS

57355-U

Supelco

SPME Fiber Assembly, 60 μm PEG, Metal Alloy (1 cm), needle size 23 ga, Manual Holder, pk of 3, purple hub

57354-U

Supelco

SPME Fiber Assembly, 60 μm PEG, Metal Alloy (1 cm), needle size 23 ga, Autosampler, pk of 3, purple hub

57328-U

Supelco

SPME Fiber Assembly, 50/30µm DVB/CAR/PDMS, StableFlex (1cm), needle size 24 ga, Manual Holder, pk of 3, plain gray hub

57302

Supelco

SPME Fiber Assembly, 7 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, green hub

57335-U

Supelco

SPME Fiber Assembly, 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, light blue hub

57348-U

Supelco

SPME Fiber Assembly, 50/30µm DVB/CAR/PDMS, StableFlex (2cm), needle size 24 ga, Autosampler, pk of 3, notched gray hub

57342-U

Supelco

SPME Fiber Assembly, 100 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, red hub

57357-U

Supelco

SPME Sampling Stand, for use with 15 mL vials

57333-U

Supelco

SPME Sampling Stand, for use with 4 mL vials

57319

Supelco

SPME Fiber Assembly, 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, black hub

57301

Supelco

SPME Fiber Assembly, 100 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, red hub

57305

Supelco

SPME Fiber Assembly, 85 μm Polyacrylate, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, white hub

57326-U

Supelco

SPME Fiber Assembly, 65 μm PDMS/DVB, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, pink hub

57311

Supelco

SPME Fiber Assembly, 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, blue hub

57359-U

Supelco

SPME Portable Field Sampler, coating PDMS/DVB

57346-U

Supelco

SPME Fiber Assembly, 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, blue hub

57318

Supelco

SPME Fiber Assembly, 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, black hub

57334-U

Supelco

SPME Fiber Assembly, 85 μm CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Manual Holder, pk of 3, light blue hub

504831

Supelco

SPME Portable Field Sampler, coating CAR/PDMS

57327-U

Supelco

SPME Fiber Assembly, 65 μm PDMS/DVB, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, pink hub

57329-U

Supelco

SPME Fiber Assembly, 50/30µm DVB/CAR/PDMS, StableFlex (1 cm), needle size 24 ga, Autosampler, pk of 3, plain gray hub

57308

Supelco

SPME Fiber Assembly, 30 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, yellow hub

57343-U

Supelco

SPME Fiber Assembly, 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, black hub

57309

Supelco

SPME Fiber Assembly, 30 μm PDMS, Fused Silica (1 cm), needle size 24 ga, Autosampler, pk of 3, yellow hub

57310-U

Supelco

SPME Fiber Assembly, 65 μm PDMS/DVB, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, blue hub

57344-U

Supelco

SPME Fiber Assembly, 75 μm CAR/PDMS, Fused Silica (1 cm), needle size 23 ga, Manual Holder, pk of 3, black hub

57341-U

Supelco

SPME Fiber Assembly, 100 μm PDMS, Fused Silica (1 cm), needle size 23 ga, Autosampler, pk of 3, red hub

57304

Supelco

SPME Fiber Assembly, 85 μm Polyacrylate, Fused Silica (1 cm), needle size 24 ga, Manual Holder, pk of 3, white hub