Comparative study between the Light Cycler and the PCR-restriction fragment length polymorphism in detecting factor V Leiden and factor II 20210G>A mutations.

To test reproducibility, speed and cost of testing for factor V Leiden and FII 20210G>A in our practice. We compared conformity, reproducibility, speed and cost using the Light Cycler (LC) and PCR-RFLP. There was 100% conformity and reproducibility. LC was faster but 23% more expensive per sample. When equipment depreciation and patient expenses are added, LC testing becomes cheaper. In our practice, LC provides fast, reproducible and cost-effective results.