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  • Variants of human seminal acrosin inhibitor (HUSI-II) which inhibit human leukocyte elastase.

Variants of human seminal acrosin inhibitor (HUSI-II) which inhibit human leukocyte elastase.

Biomedica biochimica acta (1991-01-01)
J Collins, W Taube, E Fink, A Möritz, H Fritz
ABSTRACT

A cDNA coding for human seminal inhibitor II was fused to the ompA-gene leader peptide for the expression in E. coli. The secreted protein is biologically active and correctly processed. Inhibitors of human leukocyte elastase were constructed by site-directed mutagenesis.