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Column	SUPELCOWAX 10, 15 m x 0.10 mm I.D., 0.10 µM (24343)
Oven	80C (0.3 min), 30 °C/min to 180 °C (0.5 min), 10 °C/min to 200 °C (1 min)
Carrier Gas	Hydrogen, 45 cm/sec, constant
Sample	bacterial methyl ester standards in methyl caproate, 10 mg/mL (47080-U)
Injection	0.1 µL200:1 split
Liner	2 mm I.D., straight
Inj. Temp.	250 °C
Detector	FID, 250 °C

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Analysis Note	Food-borne microbial pathogens can cause illness in humans. Because each bacterium has a unique cellular fatty acid profile, GC analysis can be used to help with identification. Analysis is performed following derivatization of cellular fatty acids to methyl esters. These are called bacterial acid methyl esters (BAMEs) instead of fatty acid methyl esters (FAMEs) to signify the bacterial source. The chromatogram shown is the analysis of a standard mix containing 26 BAME compounds. The polar nature of the column, along with the use of Fast GC column dimensions and instrument conditions, allows good peak shape and resolution in just 7 minutes.
Categories	Analytical Chromatography, GC Applications, Bacterial Acid Methyl Esters (BAMEs)
Featured Industry	Clinical
Legal Information	SUPELCOWAX is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes	Complementary Products: \r\nSyringes \r\nVials \r\nGas Purification and Gas Management \r\nInlet Liners, Glass Wool, and Specialized Hand Tools \r\nSepta and Specialized Hand Tools \r\nColumn Ferrules, Nuts, and Specialized Hand Tools
Suitability	Application for GC

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