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Column	Ascentis Si, 15 cm x 4.6 mm I.D., 5 µM particles (581512-U)
Column Temp.	30 °C
Mobile Phase	[A] hexane; [B] ethyl acetate
Gradient	10 to 30% B in 10 min; held at 30% B for 2 min
Flow Rate	1.0 mL/min
Injection	10 µL
Detector	UV, 290 nm
Sample \r\n	each compound in hexane:isopropanol (96:4)

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Analysis Note	Ascentis silica is used in the analysis of fat-soluble vitamins using a normal phase gradient. These include vitamin K 3 (menadione), vitamin E (alpha and gamma tocopherol), and vitamin D3 (cholecalciferol).
Categories	Analytical Chromatography, Vitamins and Natural products
Featured Industry	Vitamins, Nutraceuticals, and Natural Products
Legal Information	Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Suitability	Application for HPLC

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