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column	apHera NH2, 15 cm x 2 mm I.D., 5 µm particles (56400AST)
column temp.	30 °C
mobile phase	[A] water; [B] 20mM ammonium carbonate pH 9.0
gradient	100%A for 0.5 min; to 100% B in 0.1 min; held at 100% B for 6.4 min; to 100% A in 0.1 min; held at 100% A for 3 min
flow rate	0.3 mL/min
pressure	1570 psi (108 bar)
sample	50 ng/mL each compound in 50:50 methanol:water with 0.05% formic acid
injection	15 µL
detector	ESI-MS(-); MS/MS 168/63; 110/63; 180/63

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Analysis Note	An LC/MS method for analysis of glyphosate, AMPA and glufosinate using MS-compatible mobile phase on an ion-exchange pH-stable HPLC column with mass spectrometry detection is shown here.
Categories	Analytical Chromatography, Herbicides
Featured Industry	Food and Beverages
suitability	application for LC-MS

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