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Conditions

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column	BIOshell A400 Protein C4, 10 cm x 2.1 mm I. D., 3.4 µm particles (66825-U)
column temp.	50 °C
mobile phase	[A] 80:20 Water (0.1% trifluoroacetic acid): Acetonitrile (0.1% trifluoroacetic acid); [B] 20:80 Water (0.1% trifluoroacetic acid): Acetonitrile (0.1% trifluoroacetic acid)
gradient	20 to 53% B in 5 min
flow rate	0.3 mL/min
pressure	1150 psi (79 bar)
sample	Three component casein mix, 200 µg/mL, 0.1% trifluoroacetic acid
injection	10 µL
detector	UV, 215 nm

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Description

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Analysis Note	Caseins are a group of phosphoproteins found predominantly in mammalian milk. Each type of mammalian milk has a different profile of caseins (alpha, beta, etc.) based on the mammal′s diet and where it is located. Therefore, this profile can be used to authenticate where certain dairy products (i.e. cheeses) came from. A RP-HPLC method for the separation of three caseins (alpha, beta, and kappa-casein) was developed on the BIOshell A400 Protein C4 column.
Categories	Analytical Chromatography, Proteins, Peptides
Featured Industry	Life Science and Biopharma
Legal Information	BIOshell is a trademark of Sigma-Aldrich Co. LLC
Other Notes	Two isoforms of beta-casein exist in mammalian milk: A1-beta-casein and A2-beta-casein; therefore, two peaks are identified for beta-casein., Due to the heterogeneity of the standards, band broadening and extra peaks resulted in the chromatogram.
suitability	application for HPLC

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