OligoEvaluator™ for Tm Calculation & Primer Analysis
OligoEvaluator™ for DNA or RNA Primers
OligoEvaluator is a primer analysis tool for oligonucleotides. It analyses the secondary structure, primer dimer formation, and physical characteristics of oligonucleotides.
We are pleased to offer OligoEvaluator™, our online oligonucleotide sequence calculator that provides primer analysis values for PCR:
- Base count
- Molecular weight
- Extinction coefficient
- Oligo type
- µg/OD at 260 nm
- Length (base pairs)
- Primer melting temperature Tm (°C)
- GC content %
- GC clamp
- Run length (bp)
- Secondary structure
- Primer dimer check
- BLAST sequence link
OligoEvaluator™ is easy to use: select DNA or RNA, paste your sequence, and click calculate for the OligoEvaluator™ tool to return values. All reported properties are available for export to a convenient Excel template.
Compute Oligo Properties, Resuspension & Dilution
The OligoEvaluator™ consists of three modules. Choose analysis to look at the complete properties of up to 10 primers at a time. Resuspension and Dilution are separate calculator modules within OligoEvaluator™.
Calculator Advanced Features |
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Reaction Conditions
Definitions and values used in the OligoEvaluator™ calculator
How do you calculate the annealing temperature of a primer?
The OligoEvaluator™ provides melting temperature for oligos. Just enter you sequence in the calculator Analysis module, and you'll find the Tm reported in the seventh column. Annealing temperature should usually be several degrees below the Tm value. You can find more detailed information about melting temperature in our article about Oligonucleotide Melting Temperature.
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