Glycobiology Analysis Manual, 2nd Edition
Glycosylation often leads to problems in subsequent protein analysis procedures. Glycopeptides generally do not readily ionize during MS analysis leading to insufficient spectral data. Furthermore, proteolytic digestion of the native glycoprotein is often incomplete due to steric hindrance by the oligosaccharides. Removal of the carbohydrate groups from a glycoprotein prior to protein identification is preferred.
Our GlycoProfile I Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient and reproducible method to N-deglycosylate and tryptically digest protein samples in 1D or 2D polyacrylamide gel slices for subsequent MS or HPLC analysis. The procedure is suitable for Coomassie™ Blue and Colloidal Coomassie stained gels and may be used with gels silver stained and destained using our ProteoSilver™ Plus Kit (Product No. PROTSIL2). GlycoProfile Enzymatic In-gel N-Deglycosylation Kit includes the enzymes and reagents necessary for N-linked deglycosylation and tryptic digestion. The samples can then be desalted and concentrated for analysis by MALDI-TOF or electrospray MS.
Features and Benefits
Figure 1.Components of GlycoProfile I, In-Gel Deglycosylation Kit (Product No. PP0200).
The GlycoProfile Enzymatic In-solution N-Deglycosylation Kit has been optimized to provide a convenient and reproducible method to remove N-linked glycans from glycoproteins and is compatible with subsequent MALDI-TOF mass spectrometric analysis without interference from any of the reaction components. The kit contains sufficient enzyme, glycoprotein standard, and reagents, for a minimum of 20 reactions when the sample size is between one to two mg of a typical glycoprotein.
Features and Benefits
In-solution Deglycosylation
The EDEGLY Kit contains the enzymes and reagents required for deglycosylation of both N and O-linked glycans.
Features and Benefits
The NDEGLY Kit is intended for the deglycoslylation of N-linked oligosaccharides from glycoproteins under native conditions. Particular residues, due to their location in the native protein may be resistant to the traditional deglycosylation methods using PNGase F. The NDEGLY Kit utilizes endoglycosidases which are less sensitive to protein conformation than PNGase F and, therefore, more suitable for deglycosylation of native proteins.
Features and Benefits
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