form
solution
Quality Level
usage
mL sufficient for 100 mini-gels
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
concentration
10,000 × in DMSO
technique(s)
PCR: suitable
greener alternative category
storage temp.
−20°C
General description
Application
- dsDNA定量
- 聚合酶链式反应(PCR),用于DNA染色
- Comet测定技术
- 评估精子膜的完整性
- 扩增DNA 目视检查(环介导等温扩增(LAMP)法)
- 用作流式细胞术中的荧光染料
- 实时定量逆转录聚合酶链式反应(qRT-PCR),用于逆转录cDNA染色
Features and Benefits
- 用于在电泳后对琼脂糖或聚丙烯酰胺凝胶中的双链DNA染色的超灵敏染色剂
- 它还可以检测变性琼脂糖/甲醛和聚丙烯酰胺/尿素凝胶中的ssDNA和RNA,无需任何预洗涤步骤
- 在 Ames 试验中,它的致突变性比溴化乙锭小
- 它对寡核苷酸的检测灵敏度是溴化乙锭的 50-100 倍
- 适用于 DNA 数量有限的许多应用
- SYBR® Green I 与 DNA 的结合不会抑制许多常见限制性内切酶的活性,包括 Hind III 和 EcoR I
- 这种染色剂无需通过凝胶酶解和连接技术去除
- SYBR Green I 是溴化乙锭染色的绿色替代产品
Preparation Note
Legal Information
存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
201.2 °F - closed cup
flash_point_c
94 °C - closed cup
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
商品
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
实验方案
SeqPlex DNA Amplification Kit enables NGS from small or degraded DNA quantities for whole genome amplification.
Hot start dNTP protocol enhances specificity in PCR by blocking DNA polymerase nucleotide incorporation during PCR.
The SeqPlex RNA Amplification kit provides a method for amplification of total RNA or isolated mRNA prior to entry into the workflows of the commonly used deep sequencing platforms.
WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| S9430-.5ML | 04061837899140 |
| S9430-1ML | 04061837899164 |
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