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SYBR® Green 定量 RT-qPCR 试剂盒

One step SYBR® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase

别名:

one step rt qPCR, sybr green qPCR

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NACRES:
NA.55
UNSPSC Code:
41106300

主要文件

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usage

sufficient for 250 reactions

feature

dNTPs included, hotstart

technique(s)

RT-qPCR: suitable, qPCR: suitable

Quality Level

200

color

colorless

input

purified RNA

compatibility

ABI 5700, ABI 7000, ABI 7300, ABI 7500 Fast, ABI 7500, ABI 7700, ABI 7900 HT , ABI 7900 HT Fast, ABI 7900, ABI StepOne, ABI StepOnePlus, ABI ViiA 7, Bio-Rad CFX384, Bio-Rad CFX96, Bio-Rad MJ Chromo4, Bio-Rad MJ Opticon 2, Bio-Rad MJ Opticon, Bio-Rad MiniOpticon, Bio-Rad MyiQ, Bio-Rad iCycler iQ, Bio-Rad iQ5, Cepheid SmartCycler, Eppendorf® Mastercycler ep realplex2 s, Eppendorf® Mastercycler ep realplex, Illumina Eco qPCR, Qiagen Corbett Rotor-Gene 3000, Qiagen Corbett Rotor-Gene 6000, Qiagen Corbett Rotor-Gene Q, Roche LightCycler 480, Strategene Mx3000P, Strategene Mx3005P, Strategene Mx4000

detection method

SYBR® Green

shipped in

wet ice

storage temp.

−20°C

相关类别

General description

The SYBR® Green定量 RT-PCR 试剂盒将鼠白血病病毒（Moloney Murine Leukemia Virus）逆转录酶（M-MLV RT）、JumpStart™ Taq DNA聚合酶和SYBR Green I荧光染料组合成一步式 RT-PCR 试剂盒，设计用于检测基因表达。使用便捷的2X即用混合物包括M-MLV RT、SYBR Green I染料、JumpStart™ Taq DNA 聚合酶、99％纯度的脱氧核苷酸、缓冲液，玻璃钝化剂和稳定剂。 JumpStart ™Taq DNA聚合酶是一种抗体灭活的热启动酶（hot-start enzyme）。一旦反应温度达到70°C，DNA聚合酶-抗体复合物随即解离，Taq DNA聚合酶活性得以恢复。相对于手动热启动技术，这种抗体-酶复合物可轻松简便地准备，同时可降低污染风险。

Application

SYBR Green 定量 RT-PCR 试剂盒具有高特异性和再现性。 SYBR Green I是一种常用的荧光DNA结合染料，可结合所有双链DNA，并通过测量整个循环中荧光的增加来监测检测。SYBR Green I最大激活和发射峰值分别在494nm和521nm。在加入一种热启动介导tag聚合酶，JumpStart Taq后，Sigma′s基于SYBR QPCR检测的特异性被增强。 该套件已经过优化，可与板/管实时仪器和罗氏 LightCycler 毛细管仪器一起使用。 一个单独的小瓶提供了参考染料，可用于 ABI 检测系统。
SYBR® Green定量 RT-qPCR 试剂盒用于一步式逆转录聚合酶链反应（RT-PCR）测定：
  • 检测诺如病毒基因组 I 和 II 的特定基因簇
  • 定量测定基孔肯雅（CHIKV）RNA检测 mRNA 表达水平
  • 扩增从人脐静脉内皮细胞 （HUVEC）和 前列腺癌细胞中提取的总 RNA

Features and Benefits

  • 该预混液可确保反应间的一致性和可重现性
  • 缩短了制备时间，并降低了多个移液步骤造成的污染风险
  • 与手动或热启动（Hot Start）方法相比，减少了设置时间
  • JumpStart™Taq聚合酶可减少引物二聚体和非特异性产物形成。
  • SYBR® Green I染料价格便宜、易于使用、灵敏度高
  • 广泛的仪器兼容性
  • 包括一个单独的ROX参考染料瓶，用于反应标准化

Packaging

1个试剂盒足以用于每次50 μL的100次反应。

Legal Information

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护：5,994,056 和6,171,785。购买本产品，即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可，即将此等数量的产品用于购买者′内部的研究用途。未通过明示、暗示或禁止反言形式授予购买者任何其它专利主张（如美国专利号6,814,934中的仪器或系统权利要求）下的权利、执行任何专利方法的权利或开展任何形式的商业服务的权利，包括但不限于出于经济利益或其他商业考虑报告购买者′的活动结果。本品仅供研究使用。 如需用于Roche专利许可的诊断用途，需另外征得Roche许可。有关购买许可的更多信息，可联系许可总监（Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA）获取。
Eppendorf is a registered trademark of Eppendorf SE
JumpStart is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Thermo Fisher Scientific or its subsidiaries

仅试剂盒组分

产品编号
说明
  • SYBR® Green Taq ReadyMix™ for Quantitative RT-PCR 2 x 25

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书
  • P219210X PCR Buffer, Optimized for routine PCR with MgCl2 included化学品安全说明书
  • M878725 mM MgCl2 1.5 mL/vial化学品安全说明书
  • R4526Reference Dye for Quantitative PCR, 100 ×, solution .3 mL/vial化学品安全说明书

signalword

Danger

存储类别

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

wgk

WGK 3

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - ED ENV 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1

法规信息

含少量动物源组分生物产品
常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000524118
0000524118
0000498397
0000498397
0000411010

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Phui San Ho et al.
Virology journal, 7, 13-13 (2010-01-23)
Chikungunya virus (CHIKV) is a mosquito-borne alphavirus and one of the prevalent re-emerging arbovirus in tropical and subtropical regions of Asia, Africa, and Central and South America. It produces a spectrum of illness ranging from inapparent infection to moderate febrile
Caiyun Shan et al.
Molecular medicine reports, 17(4), 5300-5305 (2018-02-03)
Stroke is the most common cause of mortality worldwide. Post-stroke angiogenesis is of great significance to the treatment of strokes. The aim of the present study was to investigate the mechanism underlying the angiogenesis-promoting effect of microRNA‑126 (miR‑126)‑associated signaling pathways
Gary P Richards et al.
Applied and environmental microbiology, 70(12), 7179-7184 (2004-12-03)
Genogroup I noroviruses from five genetic clusters and genogroup II noroviruses from eight genetic clusters were detected in stool extracts using degenerate primers and single-tube, real-time reverse transcription-PCR (RT-PCR) with SYBR Green detection. Two degenerate primer sets, designated MON 431-433
Carmela R Balistreri et al.
Scientific reports, 9(1), 11028-11028 (2019-08-01)
Bicuspid aortic valve (BAV) disease is recognized to be a syndrome with a complex and multifaceted pathophysiology. Its progression is modulated by diverse evolutionary conserved pathways, such as Notch-1 pathway. Emerging evidence is also highlighting the key role of TLR4
Pei Jin Lim et al.
PLoS neglected tropical diseases, 8(2), e2661-e2661 (2014-03-04)
Chikungunya virus (CHIKV) has resulted in several outbreaks in the past six decades. The clinical symptoms of Chikungunya infection include fever, skin rash, arthralgia, and an increasing incidence of encephalitis. The re-emergence of CHIKV with more severe pathogenesis highlights its
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PCR master mix simplifies PCR/RT-PCR with components like DNA polymerase, dNTPs, MgCl2, and buffer, available commercially or DIY.

Probe QPCR for Gene-Level Expression

qPCR investigates gene expression, amplification, and alterations, crucial for tumor biology and understanding cancer genetics.

Quantitative RT-PCR

RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.

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实验方案

Reverse Transcription Protocol Using SYBR Green Dye Detection

Perform reverse transcription (RT) using a reverse transcriptase enzyme and dNTPs. Use total RNA or a gene-specific approach so that only the RNA of interest is converted to cDNA.

Primer Concentration Optimization

Primer Concentration Optimization Protocol creates reaction matrix for testing primer concentrations against various partners.

使用SYBR Green染料检测的逆转录实验方案

使用逆转录酶和dNTPs进行逆转录（RT）使用总RNA或基因特异性方法实现仅目标RNA被转化为cDNA。

引物浓度优化实验方案

引物浓度优化实验方案是一种建立反应阵列的方法。该阵列用来对照伴侣引物的不同浓度来测试每种引物的一系列浓度。

相关内容

RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

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