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Merck
CN

S1797

SOC培养基

For use in transformation

别名:

Super Optimal broth

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关于此项目

NACRES:
NA.85
UNSPSC Code:
41106200

主要文件

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grade

Molecular Biology

sterility

0.2 μm filtered

form

liquid

composition

Dextrose, 3.603 g/L , KCl, 0.186 g/L , MgSO4, 4.8 g/L , Tryptone, 20 g/L , Yeast extract, 5 g/L

technique(s)

microbiological culture: suitable

application(s)

agriculture

storage temp.

2-8°C

suitability

nonselective for Escherichia coli, nonselective for coliforms

Quality Level

500

相关类别

General description

含有分解代谢抑制物的培养基（SOC）是一种富含葡萄糖的微生物生长培养基，主要用于大肠杆菌感受态细胞转化后的复苏。 在这种营养丰富的微生物培养基中培养大肠杆菌细胞，可以抑制克隆载体上携带的lac启动子的意外激活，从而提高含有毒蛋白基因的重组质粒的转化效率。SOC培养基采用低盐配方，含有多肽、氨基酸、水溶性维生素和葡萄糖。

Application

SOC培养基可用于大肠杆菌DH5α 感受态细胞的转化。 它还被用于培养克隆后的白色菌落，以鉴定感兴趣的重组蛋白。

Features and Benefits

SOC液体提供：
  • 经过滤灭菌的即用型培养基
  • 便利的包装规格
  • 标准配方

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

法规信息

动物来源培养基
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000498782
0000498784
0000498784
0000498782
0000434973

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S Zhao et al.
Applied and environmental microbiology, 75(24), 7624-7630 (2009-10-27)
Ampicillin-resistant (Amp(r)) Salmonella enterica isolates (n = 344) representing 32 serotypes isolated from retail meats from 2002 to 2006 were tested for susceptibility to 21 other antimicrobial agents and screened for the presence of five beta-lactamase gene families (bla(CMY), bla(TEM)
Daniel C Volke et al.
Bio-protocol, 11(4), e3917-e3917 (2021-03-19)
Precise genome engineering has become a commonplace technique for metabolic engineering. Also, insertion, deletion and alteration of genes and other functional DNA sequences are essential for understanding and engineering cells. Several techniques have been developed to this end (e.g., CRISPR/Cas-assisted
Detection of Cryptosporidium parvum Oocysts on Fresh Produce Using DNA Aptamers
Iqbal A
PLoS ONE (2015)
Rebekah M Martin et al.
mSystems, 3(3) (2018-07-03)
Despite insights gained through experimental models, the set of bacterial genes important for human infection is unclear for many of our most threatening pathogens. Klebsiella pneumoniae is a leading cause of health care-associated infections (HAIs) and commonly colonizes hospitalized patients
Qiao-Yang Sun et al.
Analytical biochemistry, 394(1), 144-146 (2009-07-23)
In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which is a glucose-containing rich medium, significantly improved the transformation efficiency of a recombinant plasmid containing a toxic plant subtilase SaSBT1 cDNA.
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商品

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

细菌转化实验方案

感受态细胞生长及其转化（吸收DNA）的通用实验方案。

实验方案

Competent Cell Protocols

Technical Article on competent cells. Transformation is a process by which some bacteria take up foreign genetic material (naked DNA) from the environment.

Microbial Growth Protocols

General protocols for growth of competent cells in microbial medium.

微生物生长实验方案

在从小型培养到发酵级的不同规模下培养大肠杆菌。包括了用于启动起始培养物、悬浮细胞、单一培养物和平板噬菌体M13的实验方案。

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mRNA Synthesis

Tools for plasmid preparation, in vitro transcription, mRNA purification, and LNP formulation in mRNA-based vaccine and therapeutic development.

mRNA 合成

用于质粒制备、体外转录、mRNA 纯化和 LNP 制剂的工具，用于基于 mRNA 的疫苗和疗法开发。

Directional Cloning System

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