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文件

199583

Sigma-Aldrich

丽丝胺 ™ 绿 B

Dye content 60 %

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别名:
羊毛绿, 酸性绿 50
经验公式（希尔记法）:
C27H25N2NaO7S2
CAS号:
3087-16-9
分子量:
576.62
颜色索引号:
44090
EC 号:
221-409-2
MDL编号:
MFCD00012117
PubChem化学物质编号:
24851928
NACRES:
NA.47

形式

powder

质量水平

200

组成

Dye content, 60%

溶解性

H2O: 0.1%

λmax

633 nm

ε (消光系数)

≥10000 at 300-306 nm in water at 0.005 g/L
≥50000 at 236-242 nm in water at 0.005 g/L
≥60000 at 630-636 nm in water at 0.005 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

储存温度

room temp

SMILES string

[Na+].CN(C)c1ccc(cc1)C(=C2/C=CC(\C=C2)=[N+](/C)C)\c3c(O)c(cc4cc(ccc34)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C27H26N2O7S2.Na/c1-28(2)20-9-5-17(6-10-20)25(18-7-11-21(12-8-18)29(3)4)26-23-14-13-22(37(31,32)33)15-19(23)16-24(27(26)30)38(34,35)36;/h5-16H,1-4H3,(H2,31,32,33,34,35,36);/q;+1/p-1

InChI key

WDPIZEKLJKBSOZ-UHFFFAOYSA-M

相关类别

应用

丽丝胺绿 B 已用于血脑屏障 (BBB) 研究，并用于研究其对动物单纯疱疹病毒 1 (HSV-1) 角膜感染的影响。

生化/生理作用

丽丝胺绿又称为酸性绿 50、羊毛绿 S、食物绿 S 或绿 S，用于角膜和结膜的活体染色。染色特征与虎红染料几乎相似。 丽丝胺绿也用于染色膜损伤的上皮细胞。 在体外 ，低浓度的丽丝胺绿 B 可阻断单纯疱疹病毒 1 (HSV-1) 空斑形成。

法律信息

Lissamine is a trademark of PerkinElmer Inc.

象形图

Exclamation mark
GHS07

警示用语：

Warning

危险分类

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the ?max 199583, Lissamine™ Green B, in water? 

    The ?max in water is 633 nm.

  6. What is the solubility of Product 199583, Lissamine™ Green B?

    Lissamine Green B is soluble in water up to 0.1% in water. Other sources have stated solubility for some lots can approach 4%. Solubility in ethanol is 3 mg/mL, although this is not tested by Sigma Aldrich on a lot to lot basis. Lissamine Green B has also been reported to be soluble in Cellosolve, and in ethylene glycol.

  7. What are possible uses for Product 199583, Lissamine™ Green B?

    The Sigma Aldrich Handbook of Stains, Dyes and Indicators advises that Lissamine Green B has been substituted for Fast Green FCF without any real advantage. Lillie found it performed well as a collagen stain in Masson's trichrome and as a counter stain in the Van Gieson method. However, it was inferior when used as a component in Van Gieson's Trichrome Stain. See also Clark G, Staining Procedures, 4th Edition. (Williams & Wilkins, Baltimore, 1981), p. 120 for the Lillie modification of Masson's trichrome method.  This material is used in the determination of chlorine dioxide in solution in EPA Method 32.

  8. Has Product 199583, Lissamine™ Green B, been used for industrial applications?

    The Sigma Aldrich Handbook of Stains, Dyes and Indicators states Lissamine Green B has been used to dye wool, silk (from a sulfuric acid bath), to print wool and silk directly and to coat paper, dye leather and surface stain wood.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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Differentiation of metazoan cells requires execution of different gene expression programs but recent single-cell transcriptome profiling has revealed considerable variation within cells of seeming identical phenotype. This brings into question the relationship between transcriptome states and cell phenotypes. Additionally, single-cell
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NORN
Acta Ophthalmologica, 51, 483-491 (1973)
J Chodosh et al.
Investigative ophthalmology & visual science, 35(3), 1046-1058 (1994-03-01)
Fluorescein and rose bengal are dyes used routinely in the examination of the ocular surface. As part of an ongoing search for a superior ophthalmic dye with optimal specificity and sensitivity and a lack of interference with subsequent viral cultures
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