04-1530
抗MDM2抗体，克隆3G9
clone 3G9, from mouse
别名:
Double minute 2 protein, Mdm2 p53 binding protein homolog (mouse), Mdm2, transformed 3T3 cell double minute 2, p53 binding protein, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), Oncoprotein Mdm2, double minute 2, human homolog
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
3G9, monoclonal
Application:
immunocytochemistry
immunohistochemistry
immunoprecipitation (IP)
western blot
immunohistochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
human
Citations:
14
Technique(s):
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗MDM2抗体，克隆3G9, clone 3G9, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
3G9, monoclonal
species reactivity
human
species reactivity (predicted by homology)
mouse (based on 100% sequence homology)
packaging
antibody small pack of 25 μL
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MDM2(4193)
mouse ... Mdm2(17246)
Application
研究子类别
RNA代谢 & 结合蛋白
泛素 & 泛素代谢
RNA代谢 & 结合蛋白
泛素 & 泛素代谢
研究类别
表观遗传学&核功能
表观遗传学&核功能
免疫沉淀分析： 一个代表性批次已在一个独立实验室中进行此应用。(Changgong, L., et al. (2002). Molecular and Cellular Biology.22(21):7562-7571.)
抗MDM2抗体，克隆3G9，是一种小鼠单克隆抗体，可用于检测MDM2（也称为双微2蛋白或Mdm2 p53结合蛋白同源物） &，其已通过WB、IP、ICC、&IHC验证。
Biochem/physiol Actions
该抗体可识别MDM2。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Analysis Note
对照
MCF-7细胞裂解液
MCF-7细胞裂解液
通过蛋白质印迹对MCF-7细胞裂解液进行了评估。
蛋白质印迹分析：0.5 µg/ml该抗体可在10 µg的MCF-7细胞裂解液中检测到MDM2。
蛋白质印迹分析：0.5 µg/ml该抗体可在10 µg的MCF-7细胞裂解液中检测到MDM2。
General description
MDM2在许多肿瘤中过表达。它的主要功能是p53抑癌蛋白的泛素化和降解。当Thr-216未被磷酸化时，该单克隆抗体还可识别鼠MDM2的Thr-216周围的肽表位。SMP14还可与某些细胞角蛋白（6、14 & 16）发生交叉反应。这仅在处理某些上皮细胞时会产生此问题，而成纤维细胞不会。
~75 kDa
Immunogen
带His标签的重组蛋白，对应于人MDM2。
表位：未知
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
形式：纯化
纯化的小鼠单克隆IgGκ，溶于含有0.1 M Tris-甘氨酸（pH 7.4，150 mM NaCl）和0.05％叠氮化钠的缓冲液中。
蛋白G纯化
Preparation Note
自发运之日起，在 2-8°C 条件下可稳定保存1年
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Zhenzhen Liu et al.
Chemical biology & drug design, 85(4), 411-417 (2014-09-18)
In this article, we describe a no-wash small-molecule fluorescent probe for detecting and imaging p53-MDM2 protein-protein interaction based on an environment-sensitive fluorescent turn-on mechanism. After extensive biological examination, this probe L1 exhibited practical activity and selectivity in vitro and in
Xiaomu Li et al.
Nature communications, 7, 11740-11740 (2016-06-07)
Mitochondrial metabolism is pivotal for glucose-stimulated insulin secretion (GSIS) in pancreatic β-cells. However, little is known about the molecular machinery that controls the homeostasis of intermediary metabolites in mitochondria. Here we show that the activation of p53 in β-cells, by
Zhuohao Liu et al.
Diabetes, 67(11), 2397-2409 (2018-08-23)
Profound loss and senescence of adipose tissues are hallmarks of advanced age, but the underlying cause and their metabolic consequences remain obscure. Proper function of the murine double minute 2 (MDM2)-p53 axis is known to prevent tumorigenesis and several metabolic
Lenka Kyjacova et al.
Oncogene, 40(47), 6494-6512 (2021-10-07)
Expression of the immediate-early response gene IER2 has been associated with the progression of several types of cancer, but its functional role is poorly understood. We found that increased IER2 expression in human melanoma is associated with shorter overall survival
Javier Octavio Mejía-Hernández et al.
Cancers, 14(16) (2022-08-27)
Metastatic prostate cancer is a lethal disease in patients incapable of responding to therapeutic interventions. Invasive prostate cancer spread is caused by failure of the normal anti-cancer defense systems that are controlled by the tumour suppressor protein, p53. Upon mutation
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