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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
NL41, monoclonal
Species reactivity:
human
Application:
immunocytochemistry
multiplexing
western blot
multiplexing
western blot
Technique(s):
immunocytochemistry: suitable
multiplexing: suitable
western blot: suitable
multiplexing: suitable
western blot: suitable
Citations:
8
Uniprot accession no.:
产品名称
抗磷酸CENP-A(Ser7)抗体,克隆NL41,兔单克隆, culture supernatant, clone NL41, Upstate®
biological source
rabbit
antibody form
culture supernatant
antibody product type
primary antibodies
clone
NL41, monoclonal
species reactivity
human
packaging
antibody small pack of 25 μL
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
multiplexing: suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
phosphorylation (pSer7)
Quality Level
Gene Information
human ... CENPA(1058)
Analysis Note
对照
从秋水仙酰胺阻滞的 HeLa 细胞中酸提取的蛋白质
从秋水仙酰胺阻滞的 HeLa 细胞中酸提取的蛋白质
通过免疫印迹法对从秋水仙酰胺阻滞的 HeLa 细胞中酸提取的蛋白质进行了常规评估(目录号 17-306)
Application
抗磷酸 CENP-A(Ser7)抗体,克隆 NL41 是用于检测磷酸 CENP-A(Ser7)的高质量兔单克隆抗体,&已在 Mplex、WB、ICC 中进行了验证。
研究子类别
细胞周期,DNA 复制&修复
细胞周期,DNA 复制&修复
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
CENP-A 在丝氨酸 7 上被磷酸化
其他未经测试。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
CENP-A 是仅在着丝粒处发现的组蛋白 H3 的变体形式。 在有丝分裂期间,它在丝氨酸 7 处被磷酸化。
〜17kDa
着丝粒蛋白 A(CENP-A)是一种 17 kDa 着丝粒特异性组蛋白变体,与组蛋白 H3 的 C 端具有 62% 的氨基酸同源性。它位于着丝粒中,在着丝粒特异性染色质的形成中起着关键作用。敲除组蛋白 H3 的 CENP-A 结合域的研究表明,CENP-A 可能是将 α-卫星 DNA 包装到初级染色结构中的组蛋白 H3 的替代品。CENP-A 是着丝粒的特异性核小体的形成所必需的,这暗示着 CENP-A 是着丝粒特异性的表观遗传标记。
Immunogen
含有 RRpSRK 序列的肽,其中 pS 是对应于人 CENP-A 氨基酸 7 的磷酸丝氨酸(着丝粒蛋白 A)
Other Notes
替代:05-792
Physical form
0.05% 叠氮化钠中的培养上清液
Preparation Note
自收到之日起,在-20°C 条件下可稳定保存 1 年。
为最大程度地回收产品,在打开瓶盖之前先将小瓶离心。避免反复冻融循环,以免损坏 IgG 和影响产品性能。
为最大程度地回收产品,在打开瓶盖之前先将小瓶离心。避免反复冻融循环,以免损坏 IgG 和影响产品性能。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
Anna P Baron et al.
eLife, 5 (2016-02-18)
The kinase Bub1 functions in the spindle assembly checkpoint (SAC) and in chromosome congression, but the role of its catalytic activity remains controversial. Here, we use two novel Bub1 inhibitors, BAY-320 and BAY-524, to demonstrate potent Bub1 kinase inhibition both
Aisling O'Connor et al.
Biology open, 5(1), 11-19 (2015-12-20)
During mitotic arrest induced by microtubule targeting drugs, the weakening of the spindle assembly checkpoint (SAC) allows cells to progress through the cell cycle without chromosome segregation occurring. PLK1 kinase plays a major role in mitosis and emerging evidence indicates
Grzegorz Dobrynin et al.
Journal of cell science, 124(Pt 9), 1571-1580 (2011-04-14)
During exit from mitosis in Xenopus laevis egg extracts, the AAA+ ATPase Cdc48/p97 (also known as VCP in vertebrates) and its adapter Ufd1-Npl4 remove the kinase Aurora B from chromatin to allow nucleus formation. Here, we show that in HeLa
Asha Recino et al.
The Biochemical journal, 430(2), 207-213 (2010-07-16)
RASSF7, a member of the N-terminal Ras association domain family, has increased expression in various cancers and, on the basis of our previous work in Xenopus embryos, may be a regulator of mitosis. In the present study, we address, for
Anna De Antoni et al.
The Journal of cell biology, 199(2), 269-284 (2012-10-17)
By phosphorylating Thr3 of histone H3, Haspin promotes centromeric recruitment of the chromosome passenger complex (CPC) during mitosis. Aurora B kinase, a CPC subunit, sustains chromosome bi-orientation and the spindle assembly checkpoint (SAC). Here, we characterize the small molecule 5-iodotubercidin
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