05-1000X
抗-磷酸丝氨酸抗体，克隆4A4
clone 4A4, Upstate®, from mouse
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
4A4, monoclonal
Species reactivity:
vertebrates
Application:
ELISA
flow cytometry
immunofluorescence
immunohistochemistry
western blot
flow cytometry
immunofluorescence
immunohistochemistry
western blot
Technique(s):
ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
Citations:
12
产品名称
抗-磷酸丝氨酸抗体，克隆4A4, clone 4A4, Upstate®, from mouse
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
4A4, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable
flow cytometry: suitable
immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
phosphorylation (pSer)
Quality Level
General description
取决于能检测到的丝氨酸磷酸化蛋白的分子量。
将蛋白质磷酸化鉴定为一种调节机制，起源于1950年代中期Fischer和Krebs的研究，后来使他们获得了1992年的诺贝尔奖。它是调节多种细胞过程的主要机制，包括细胞分裂，蛋白质合成，转录调节和神经传递。任何给定底物的稳态磷酸化均受激酶和磷酸酶的相反活性控制。现在认为，所有真核细胞蛋白中有三分之一被磷酸化，并且所有磷酸化事件中的大多数发生在丝氨酸和苏氨酸残基上（大于95%）。
Analysis Note
通过免疫印迹分析对来自Calyculin A/Okadaic处理的人A431癌细胞的裂解液进行常规评估。
Application
抗磷酸丝氨酸抗体，克隆4A4是用于WB、IF、FC、IH(P)、ELISA的抗磷酸丝氨酸抗体。
研究亚类
常规翻译后修改
常规翻译后修改
研究类别
信号传导
信号传导
Biochem/physiol Actions
所有物种中的丝氨酸磷酸化蛋白
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Immunogen
磷酸丝氨酸与KLH偶联
Physical form
50 µL 含0.05%叠氮化钠和30%甘油的PBS。
形式：纯化
蛋白G-琼脂糖层析
Preparation Note
自运送之日起在-20°C下保存2年。
为了最大程度地回收产品，需在取下盖子之前将原始样品管进行离心。如果产品意外发生了冻融，可在2-8°C下以13,000 x g离心10分钟。
为了最大程度地回收产品，需在取下盖子之前将原始样品管进行离心。如果产品意外发生了冻融，可在2-8°C下以13,000 x g离心10分钟。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
Ying Yang et al.
Chemistry & biodiversity, 19(11), e202200333-e202200333 (2022-09-24)
N6-Methyladenosine (m6A), one of the post-transcriptional modifications of RNA, is important in hepatocellular carcinoma (HCC). However, the mechanism of its regulation remains elusive. We here show that exposure of HCC cells to sulfatide significantly reduced the total mRNA m6A modification.
SCF(Fbxw15) mediates histone acetyltransferase binding to origin recognition complex (HBO1) ubiquitin-proteasomal degradation to regulate cell proliferation.
Zou, C; Chen, Y; Smith, RM; Snavely, C; Li, J; Coon, TA; Chen, BB; Zhao, Y; Mallampalli, RK
The Journal of Biological Chemistry null
Avital Mendelson et al.
JCI insight, 4(16) (2019-08-23)
The complex process of platelet formation originates with the hematopoietic stem cell, which differentiates through the myeloid lineage, matures, and releases proplatelets into the BM sinusoids. How formed platelets maintain a low basal activation state in the circulation remains unknown.
Phosphorylation state-dependent modulation of spinal glycine receptors alleviates inflammatory pain.
Mario A Acuña et al.
The Journal of clinical investigation, 126(7), 2547-2560 (2016-06-09)
Diminished inhibitory neurotransmission in the superficial dorsal horn of the spinal cord is thought to contribute to chronic pain. In inflammatory pain, reductions in synaptic inhibition occur partially through prostaglandin E2- (PGE2-) and PKA-dependent phosphorylation of a specific subtype of
Vic Hart et al.
Journal of cancer research and clinical oncology, 147(10), 2893-2912 (2021-06-18)
In this study, two novel alternative splice variants of HER2, named HER2-PI9 and HER2-I12, were identified in breast cancer cell lines and breast tumour tissues. Whilst HER2-P19 arises from the inclusion of an 117 bp cassette-exon of intron 9 of HER2
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