05-1000X
抗-磷酸丝氨酸抗体，克隆4A4
clone 4A4, Upstate®, from mouse
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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
4A4, monoclonal
Species reactivity:
vertebrates
Application:
ELISA, FACS, IF, IHC, WB
Citations:
12
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
4A4, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Upstate®
technique(s)
ELISA: suitable, flow cytometry: suitable, immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG1
shipped in
wet ice
target post-translational modification
phosphorylation (pSer)
Quality Level
General description
取决于能检测到的丝氨酸磷酸化蛋白的分子量。
将蛋白质磷酸化鉴定为一种调节机制，起源于1950年代中期Fischer和Krebs的研究，后来使他们获得了1992年的诺贝尔奖。它是调节多种细胞过程的主要机制，包括细胞分裂，蛋白质合成，转录调节和神经传递。任何给定底物的稳态磷酸化均受激酶和磷酸酶的相反活性控制。现在认为，所有真核细胞蛋白中有三分之一被磷酸化，并且所有磷酸化事件中的大多数发生在丝氨酸和苏氨酸残基上（大于95%）。
Immunogen
磷酸丝氨酸与KLH偶联
Application
抗磷酸丝氨酸抗体，克隆4A4是用于WB、IF、FC、IH(P)、ELISA的抗磷酸丝氨酸抗体。
研究亚类
常规翻译后修改
常规翻译后修改
研究类别
信号传导
信号传导
Biochem/physiol Actions
所有物种中的丝氨酸磷酸化蛋白
Physical form
50 µL 含0.05%叠氮化钠和30%甘油的PBS。
形式：纯化
蛋白G-琼脂糖层析
Preparation Note
自运送之日起在-20°C下保存2年。
为了最大程度地回收产品，需在取下盖子之前将原始样品管进行离心。如果产品意外发生了冻融，可在2-8°C下以13,000 x g离心10分钟。
为了最大程度地回收产品，需在取下盖子之前将原始样品管进行离心。如果产品意外发生了冻融，可在2-8°C下以13,000 x g离心10分钟。
Analysis Note
通过免疫印迹分析对来自Calyculin A/Okadaic处理的人A431癌细胞的裂解液进行常规评估。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
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