biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
bFM-2, monoclonal
species reactivity
mouse, bovine, human, rat
manufacturer/tradename
Upstate®
technique(s)
immunohistochemistry: suitable, radioimmunoassay: suitable, western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... FGF2(2247)
mouse ... Fgf2(281161), Fgf2(14173)
rat ... Fgf2(54250)
General description
17.5 kDa
FGF碱性(FGF2)是成纤维细胞生长因子(FGF)家族的成员。FGF家族成员结合肝素,并具有广泛的促有丝分裂和血管生成活性。FGF2参与多种生物学过程中,例如肢体和神经系统发育、伤口愈合和肿瘤生长。FGF2 mRNA包含多个聚腺苷酸化位点,并交替从AUG和非 AUG(CUG)起始密码子进行翻译,产生具有不同特性的五个不同亚型。CUG 起始的亚型位于细胞核中,负责内分泌作用,而 AUG 起始的形式主要存在于细胞质中,并负责该 FGF 的旁分泌和自分泌作用。
Immunogen
纯化牛脑碱性FGF(FGF-2,HBGF-2)。克隆bFM-2。
Application
使用该抗FGF-2/碱性FGF抗体,克隆bFM-2检测FGF-2/碱性FGF,已验证可用于IH、RIA & WB。
免疫组化:
该抗体的先前批次已显示可对用冰冷的乙醇:乙酸[95:5]固定的冷冻脑切片进行免疫染色。该抗体已经以10 μg/mL用于染色石蜡包埋切片(Matsuzaki, K., 1989)。
该抗体的先前批次已显示可对用冰冷的乙醇:乙酸[95:5]固定的冷冻脑切片进行免疫染色。该抗体已经以10 μg/mL用于染色石蜡包埋切片(Matsuzaki, K., 1989)。
研究子范畴
生长因子 & 受体
生长因子 & 受体
研究类别
信号传导
信号传导
Biochem/physiol Actions
该高特异性抗体不会与牛酸性FGF(FGF-1,HBGF-1)发生交叉反应。
注意:该抗体可与天然和热灭活的碱性FGF发生交叉反应,但不会中和生物活性(Uteza,Y.,1999)。Upstate推荐抗FGF-2/碱性FGF,克隆bFM-1(中和)(目录号05-117)用于中和活性。
注意:该抗体可与天然和热灭活的碱性FGF发生交叉反应,但不会中和生物活性(Uteza,Y.,1999)。Upstate推荐抗FGF-2/碱性FGF,克隆bFM-1(中和)(目录号05-117)用于中和活性。
Physical form
形式:纯化
硫酸铵沉淀和DEAE-纤维素层析
Preparation Note
自收到之日起在-20ºC可稳定保存1年。
Analysis Note
已通过碱性FGF(目录#01-114)进行评估。
蛋白质印迹分析:
0.5-2 μg/mL该抗体检测100 ng FGF-2/碱性FGF, 不含载体(目录# 01-114)。
蛋白质印迹分析:
0.5-2 μg/mL该抗体检测100 ng FGF-2/碱性FGF, 不含载体(目录# 01-114)。
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Immunohistochemical localization of cytokines in nevi.
M G Fleming, S F Howe, A G Candel
The American Journal of Dermatopathology null
Immunolocalization of acidic and basic fibroblast growth factors in porcine uterine and conceptus tissues
Gupta, A., et al
Biology of Reproduction, 56, 1527-1536 (1997)
Y Uteza et al.
Proceedings of the National Academy of Sciences of the United States of America, 96(6), 3126-3131 (1999-03-17)
We developed an experimental approach with genetically engineered and encapsulated mouse NIH 3T3 fibroblasts to delay the progressive degeneration of photoreceptor cells in dark-eyed Royal College of Surgeons rats. These xenogeneic fibroblasts can survive in 1. 5-mm-long microcapsules made of
Immunolocalization and temporal distribution of cytokine expression during the development of vein graft intimal hyperplasia in an experimental model.
P L Faries, M L Marin, F J Veith, J A Ramirez, W D Suggs, R E Parsons, L A Sanchez, R T Lyon
Journal of Vascular Surgery null
Stefanie J Kirwin et al.
Investigative ophthalmology & visual science, 50(11), 5396-5404 (2009-05-29)
Inflammation is thought to play a role in disease progression and vision loss in diabetic retinopathy (DR). However, the level of inflammation and the role of cytokines and growth factors in the early stages of this disease are poorly understood.
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