biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
P3D1, monoclonal
species reactivity
mouse, human
species reactivity (predicted by homology)
rat
technique(s)
ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG2a
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... ENG(2022)
mouse ... Eng(13805)
rat ... Eng(497010)
General description
~71 kDa
内皮糖蛋白是由95 kDa亚基组成的同型二聚体整合膜糖蛋白。内皮糖蛋白是TGF-B受体系统的一个组分,其作为辅助受体起作用,影响转化生长因子β(TGF-β)家族配体与信号受体的结合。内皮糖蛋白以高亲和力结合TGF-B1和TGF-B3,但未结合TGF-b2。内皮糖蛋白由胎盘的血管内皮细胞和合体滋养层细胞表达,而间质成纤维细胞则弱表达。它也由U937细胞和活化的巨噬细胞表达。内皮糖蛋白的表达在经历血管生成的组织(例如在肿瘤中)或在伤口愈合或皮肤炎症的情况下在活化的内皮上上调。
Immunogen
人静息脐静脉内皮细胞。
Application
免疫组化:用于丙酮或多聚甲醛固定的组织。
ELISA: 先前批次的抗体用于ELISA。
免疫沉淀: 先前批次的抗体用于IP。
免疫细胞化学: 先前批次的抗体用于IC。
流式细胞术: 先前批次的抗体用于FC。
终端用户需自行确定最佳的工作稀释度。
ELISA: 先前批次的抗体用于ELISA。
免疫沉淀: 先前批次的抗体用于IP。
免疫细胞化学: 先前批次的抗体用于IC。
流式细胞术: 先前批次的抗体用于FC。
终端用户需自行确定最佳的工作稀释度。
抗内皮糖蛋白抗体,克隆P3D1是抗内皮糖蛋白的抗体,用于WB,IH,IC,IP,FC,ELISA。
研究子范畴
生长因子 & 受体
生长因子 & 受体
研究类别
信号传导
信号传导
Biochem/physiol Actions
与人内皮糖蛋白反应。
Physical form
形式:纯化
纯化
纯化小鼠单克隆IgG2a,溶于0.02 M磷酸盐缓冲液、0.25 M氯化钠、0.1%叠氮化钠,pH 7.6
Preparation Note
自收到之日起,在2-8°C下可稳定保存1年。
Analysis Note
对照
Jurkat细胞裂解液
Jurkat细胞裂解液
通过蛋白质印迹在Jurkat裂解液中进行了常规评估。
蛋白质印迹分析: 该批次以1:500的稀释度在10 μg Jurkat裂解液中检测到ENDOGLIN。
蛋白质印迹分析: 该批次以1:500的稀释度在10 μg Jurkat裂解液中检测到ENDOGLIN。
Other Notes
替代:MAB2152
浓度:请参考批次特异性浓缩物的分析证书。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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