05-1588
抗-U1-70K抗体，克隆9C4.1
clone 9C4.1, from mouse
别名:
small nuclear ribonucleoprotein 70kDa (U1), U1 small nuclear ribonucleoprotein 70 kDa, small nuclear ribonucleoprotein 70kDa (RNP antigen), U1 snRNP 70 kDa
产品名称
抗-U1-70K抗体，克隆9C4.1, clone 9C4.1, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
9C4.1, monoclonal
species reactivity
rat, mouse, human
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... SNRNP70(6625)
Analysis Note
对照
HeLa细胞裂解液
HeLa细胞裂解液
通过蛋白质印迹法在HeLa细胞裂解液中进行了评估。
蛋白质印迹分析：0.5 µg/mL该抗体可在10 µg HeLa细胞裂解液中检测到U1-70K。
蛋白质印迹分析：0.5 µg/mL该抗体可在10 µg HeLa细胞裂解液中检测到U1-70K。
Application
使用在WB、ICC中验证的抗U1-70K抗体，克隆9C4.1（小鼠单克隆抗体）检测U1-70K，也称为核小核糖核蛋白70kDa（U1）。
免疫细胞化学分析： 已显示该抗体代表性批次的1:500稀释液可在NIH/3T3、A431和HeLa细胞中检测到U1-70K。
研究子范畴
RNA代谢&结合蛋白
RNA代谢&结合蛋白
研究类别
表观遗传学&核功能
表观遗传学&核功能
Biochem/physiol Actions
该抗体可识别C端结构域处的U1-70K。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
U1小核糖核蛋白70 kDa（U1-70K）是在U1 snRNP核心中发现的三种特定蛋白之一，其中包括在所有U snRNP中发现的至少七种核心蛋白和三种特定蛋白：U1-70K，U1-A和U1-C。U1-70K通过将U1-snRNA的环I区与其RPM（RNA识别基序）结合域结合，参与介导前mRNA剪接。此外，其RNA结合结构域U1-70K还包含两个富含精氨酸的区域，这些区域带有酸性或丝氨酸残基，被认为具有反式作用。选择性剪接产生4种已知的亚型。截短形式的U1-70K无法结合RNA。
计算值 52 kDa 观测值 ~57 kDa
Immunogen
对应于人U1-70K的C末端结构域的重组蛋白。
表位：C端结构域
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
形式：纯化
纯化的小鼠单克隆IgG2aκ，溶于含有0.1 M Tris-甘氨酸（pH 7.4，150 mM NaCl）和0.05％叠氮化钠的缓冲液中。
蛋白G纯化
Preparation Note
自收到之日起，在2-8°C条件下可稳定保存1年。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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