05-212

抗-PI3激酶抗体，p85，N-SH3，克隆AB6

Name: 抗-PI3激酶抗体，p85，N-SH3，克隆AB6
Brand: MM

clone AB6, Upstate^®, from mouse

别名:

PI3-kinase p85 subunit alpha, PtdIns-3-kinase p85-alpha, phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 1 (p85

alpha), phosphatidylinositol 3-kinase, regulatory, 1, phosphatidylinositol 3-kinase-associated p-85 alpha, phosphoinos

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关于此项目

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Conjugate:

unconjugated

Clone:

AB6, monoclonal

Application:

ICC, IP, WB

Citations:

主要文件

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biological source

mouse

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

AB6, monoclonal

species reactivity

human, rat, mouse

manufacturer/tradename

Upstate^®

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_181504

UniProt accession no.

P27986

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... PIK3R1(5295)

General description

85 kDa

磷脂酰肌醇3激酶（PI3激酶）是一种脂质激酶，据信在生长信号通路中磷酸化第二信使。PI3K是催化110 kDa（p110）和调节85 kDa（p85、p50或p55）亚基的异源二聚体。p85单元由两个密切相关的蛋白质组成：与催化亚基紧密结合的p85 α和功能未知的p85 β。它们有62%的氨基酸序列相同。p85 α缺乏PI3K激酶活性，但可以作为PI3K和配体激活受体之间的纽带，成为催化亚基p110的调节剂。这两种亚型都与活化的受体结合，并作为酪氨酸激酶底物。

Immunogen

在大肠杆菌中表达的重组人p85 α PI3激酶。克隆AB6。

Application

免疫细胞化学：
10 µg/mL该抗体的先前批次已被用于免疫细胞化学。

免疫沉淀：
先前批次使用多克隆PI3激酶抗体（目录号06-195）进行免疫印迹，从500 µg A431 RIPA裂解液中免疫沉淀出4 µg PI3 激酶。

研究子类别
PI3K、Akt & mTOR信号传导

激酶&磷酸酶

研究类别
信号传导

该抗-PI3激酶抗体，p85，N-SH3，克隆AB6经验证可用于IC、IP、WB中PI3激酶的检测。

Biochem/physiol Actions

该抗体能识别PI3激酶的p85 α亚基；不能识别 p85 β亚基。

Physical form

形式：纯化

纯化的小鼠单克隆IgG1κ，溶于含有PBS（pH 7.2）和50%甘油的缓冲液中。

蛋白A层析

Preparation Note

自收到之日起，在 -20°C 条件下可稳定保存 1 年。

处理建议：
收到后，在取下瓶盖之前，将小瓶离心并轻轻混合溶液。分装到微量离心管中，并储存于-20ºC。避免反复冻融，其可能会破坏IgG并影响产品性能。注意：冷藏室温度变化至低于-20ºC时可能导致-含甘油的溶液在储存过程中冻结。

Analysis Note

对照
阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。加入2.5µL 2-巯基乙醇/100µL裂解液，煮沸5分钟以减少制备量。在微凝胶每个泳道上样20µg经还原的裂解液。

通过蛋白质印迹法对人A431细胞、大鼠L6成肌细胞的RIPA裂解液进行常规评估。

蛋白质印迹分析：
0.5-2 µg/mL该批次在人A431细胞的RIPA裂解液中检测到PI3激酶；先前批次在大鼠L6成肌细胞的RIPA裂解液中检测到PI3激酶。

Other Notes

替代：04-403

浓度：请参考批次特异性浓缩物的检验报告。

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Differential activation of phospholipase C-gamma 1 and mitogen-activated protein kinase in naive and antigen-primed CD4 T cells by the peptide/MHC ligand

Ericsson, P. O., et al

Journal of immunology (Baltimore, Md. : 1950), 156, 2045-2053 (1996)

Involvement of the PI 3-kinase signaling pathway in progression of colon adenocarcinoma.

Kianoush Khaleghpour et al.

Carcinogenesis, 25(2), 241-248 (2003-10-28)

The phosphoinositide 3-kinase (PI 3-kinase) signaling pathway has been shown to play a pivotal role in intracellular signal transduction pathways involved in cell growth, cellular transformation and tumorigenesis. Analysis of several colon adenocarcinoma cell lines indicates that the PI 3-kinase

Activation of c-Met and upregulation of CD44 expression are associated with the metastatic phenotype in the colorectal cancer liver metastasis model.

Victoria A Elliott et al.

PloS one, 9(5), e97432-e97432 (2014-05-16)

Liver metastasis is the most common cause of death in patients with colorectal cancer. Despite extensive research into the biology of cancer progression, the molecular mechanisms that drive colorectal cancer metastasis are not well characterized. HT29 LM1, HT29 LM2, HT29

Identification of a Novel Invasion-Promoting Region in Insulin Receptor Substrate 2.

Jose Mercado-Matos et al.

Molecular and cellular biology, 38(14) (2018-04-25)

Although the insulin receptor substrate (IRS) proteins IRS1 and IRS2 share considerable homology and activate common signaling pathways, their contributions to breast cancer are distinct. IRS1 has been implicated in the proliferation and survival of breast tumor cells. In contrast

Structure of 85 kDa subunit of human phosphatidylinositol 3-kinase analyzed by using monoclonal antibodies

Tanaka, S., et al

Japanese Journal of Cancer Research, 84, 279-289 (1993)

查看所有相关文献

抗-PI3激酶抗体，p85，N-SH3，克隆AB6

clone AB6, Upstate®, from mouse

选择尺寸

关于此项目

主要文件

属性

biological source

conjugate

antibody form

antibody product type

clone

species reactivity

manufacturer/tradename

technique(s)

isotype

NCBI accession no.

UniProt accession no.

shipped in

target post-translational modification

Quality Level

Gene Information

相关类别

说明

General description

Immunogen

Application

Biochem/physiol Actions

Physical form

Preparation Note

Analysis Note

Other Notes

Legal Information

Disclaimer

安全信息

存储类别

wgk

flash_point_f

flash_point_c

文件

分析证书(COA)

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clone AB6, Upstate^®, from mouse