05-636-AF555
抗磷酸化-H2A.X（Ser139），克隆JBW301，Alexa Fluor™ 555偶联物
clone JBW301, from mouse, ALEXA FLUOR™ 555
别名:
H2AXS139P, Histone H2A.X (phospho S139)
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
ALEXA FLUOR™ 555
Clone:
JBW301, monoclonal
Application:
immunocytochemistry
Species reactivity:
human
Citations:
10
Technique(s):
immunocytochemistry: suitable
Uniprot accession no.:
产品名称
抗磷酸化-H2A.X（Ser139），克隆JBW301，Alexa Fluor™ 555偶联物, clone JBW301, from mouse, ALEXA FLUOR™ 555
biological source
mouse
conjugate
ALEXA FLUOR™ 555
antibody form
purified antibody
antibody product type
primary antibodies
clone
JBW301, monoclonal
species reactivity
human
species reactivity (predicted by homology)
vertebrates (based on 100% sequence homology)
technique(s)
immunocytochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer139)
Quality Level
Gene Information
human ... H2AX(3014)
Application
抗磷酸-H2A.X（Ser139），克隆JBW301，Alexa Fluor™ 555偶联物是一种抗磷酸-H2A.X（Ser139）的抗体，用于免疫细胞化学。
染色质免疫沉淀分析：该抗体（目录号05-636）的代表性批次用于通过ChIP检测磷酸组蛋白 H2A.X（Ser139）（Meier, A. et al. (2007) EMBO J., 26:2707-18).
蛋白质印迹分析：该抗体非偶联形态的0.05-1 μg/mL代表性批次（目录号05-636）在0.5 μM星形孢菌素（目录号19-123）处理过的Jurkat细胞中酸提取的组蛋白裂解液中检测到磷酸化组蛋白H2A.X（Ser139）。
蛋白质印迹分析：该抗体非偶联形态的0.05-1 μg/mL代表性批次（目录号05-636）在0.5 μM星形孢菌素（目录号19-123）处理过的Jurkat细胞中酸提取的组蛋白裂解液中检测到磷酸化组蛋白H2A.X（Ser139）。
研究子类别
组蛋白
组蛋白
研究类别
表观遗传学&核功能
表观遗传学&核功能
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Analysis Note
通过免疫细胞化学在HeLa细胞+/- 星形孢菌素处理中进行评估。
免疫细胞化学分析：该抗体的1:100稀释液在HeLa细胞+/- 星形孢菌素处理中检测到磷酸组蛋白 H2A.X（Ser139）。
免疫细胞化学分析：该抗体的1:100稀释液在HeLa细胞+/- 星形孢菌素处理中检测到磷酸组蛋白 H2A.X（Ser139）。
General description
组蛋白H2A.X，也称H2A/X或H2A.X，由基因名称H2AFX和H2AX编码，是组蛋白H2A的变体，与基因组DNA相似。但是，组蛋白在结构上与H2A家族的其他成员不同，因为它具有一个C末端尾巴，该尾巴包含Ser139残基，该残基响应于双链DNA的断裂而被磷酸化。H2A.X的磷酸化是由ATM/ATR蛋白介导的快速过程。作为组蛋白H2A家族的成员，组蛋白H2A.x（H2A.x）是在核小体子集中替代常规H2A的变体组蛋白H2A。H2A.x参与核DNA上双链断裂（DSB）损伤的DNA修复。双链DNA断裂后，H2A.x在丝氨酸139处被ATM激酶迅速磷酸化，并变成γ-H2AFX。该磷酸化步骤可以从DSB的实际位点延伸至数千个核小体，并且可以标记周围的染色质，以募集DNA损伤信号传导和修复（1）所需的蛋白质。作为严重DNA损伤引起的细胞凋亡过程中翻译后修饰的一部分，磷酸化H2A.x的高表达被认为是细胞凋亡的准确指标。
观测值17 kDa
Immunogen
对应于磷酸化组蛋白H2A.X（Ser139）的线性肽。
Other Notes
浓度：请参考特定批次的数据表。
Physical form
纯化的小鼠单克隆IgG1在含有15 mg/mL BSA和0.1%叠氮化钠的PBS缓冲液中。
蛋白G纯化
Preparation Note
自收到之日起，在2-8°C条件下可稳定保存1年。
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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