生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
6F-H2, monoclonal
种属反应性
human
制造商/商品名称
Upstate®
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... WT1(7490)
一般描述
55-60kDa
免疫原
6His标记融合蛋白,对应人WT1(维尔姆斯瘤)的1-181位残基。
应用
抗-WT1抗体(克隆6F-H2)是一种用于检测WT1(又称维尔姆斯瘤)的小鼠单克隆抗体,目前已通过WB、ICC & IHC应用验证。
生化/生理作用
WT1
外形
形式:纯化
分析说明
已通过免疫印迹对Jurkat和Raji细胞的RIPA裂解液进行常规评估
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
Toru Sakairi et al.
American journal of physiology. Renal physiology, 298(3), F557-F567 (2009-12-04)
Evidence suggests that loss of podocytes into urine contributes to development of glomerular diseases; shed podocytes are frequently viable and proliferate in culture conditions. To determine the phenotypic characteristics of viable urinary cells derived from human subjects, we established long-term
Embryonic Stem Cells Derived Kidney Organoids as Faithful Models to Target Programmed Nephrogenesis.
Zenglai Tan et al.
Scientific reports, 8(1), 16618-16618 (2018-11-11)
The kidney is a complex organ that is comprised of thousands of nephrons developing through reciprocal inductive interactions between metanephric mesenchyme (MM) and ureteric bud (UB). The MM undergoes mesenchymal to epithelial transition (MET) in response to the signaling from
Caihua Xu et al.
PloS one, 8(8), e68837-e68837 (2013-08-13)
The Wilms' tumor suppressor gene (WT1) has been identified as an oncogene in many malignant diseases such as leukaemia, breast cancer, mesothelioma and lung cancer. However, the role of WT1 in non-small-cell lung cancer (NSCLC) carcinogenesis remains unclear. In this
Jing Zhou et al.
Biology open, 7(5) (2018-04-19)
Mesodermal populations can be generated in vitro from mouse embryonic stem cells (mESCs) using three-dimensional (3-D) aggregates called embryoid bodies or two-dimensional (2-D) monolayer culture systems. Here, we investigated whether Brachyury-expressing mesodermal cells generated using 3-D or 2-D culture systems
Lars Fuhrmann et al.
International journal of molecular sciences, 22(8) (2021-05-01)
A growing body of evidence suggests that low nephron numbers at birth can increase the risk of chronic kidney disease or hypertension later in life. Environmental stressors, such as maternal malnutrition, medication and smoking, can influence renal size at birth.
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