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Merck
CN

05-844

抗-PLK1抗体，克隆35-206

clone 35-206, Upstate®, from mouse

别名:

Polo-like kinase 1, Serine/threonine-protein kinase 13, cell cycle regulated protein kinase, polo (Drosophia)-like kinase, polo like kinase, polo like kinase, polo-like kinase (Drosophila), polo-like kinase 1 (Drosophila)

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

主要文件

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产品名称

抗-PLK1抗体，克隆35-206, clone 35-206, Upstate®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

35-206, monoclonal

species reactivity

rat, mouse, human

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG2b

NCBI accession no.

NP_005021

UniProt accession no.

P53350

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100
400

Gene Information

human ... PLK1(5347)
mouse ... Plk1(18817)

相关类别

Analysis Note

对照
阳性抗原对照：货号12-305，3T3/A31裂解液。加入2.5 μL 2-巯基乙醇/100 μL裂解液，煮沸5分钟以还原样本。在微凝胶每个泳道上样20 μg经还原的裂解液。
已通过蛋白质印迹对3T3/A31细胞的RIPA裂解液进行了评估。

蛋白质印迹分析：0.5-2 μg/mL该抗体在3T3/A31细胞的RIPA裂解液中检测到PLK1。

Application

免疫沉淀：一个独立实验室报道，一个先前批次的该抗体可用于PLK1的免疫沉淀。

免疫组织/免疫细胞化学： 一个独立实验室报道，1 μg/mL的先前批次的该抗体在多聚甲醛处理、Triton X-100 固定的细胞中显示出PLK1 的阳性免疫染色。
抗-PLK1抗体（克隆35-206）可检测PLK1水平 & 已发布 & 经验证可用于WB、IP、IC、IH。
研究子类别
细胞周期，DNA 复制&修复
研究类别
表观遗传学&核功能

Biochem/physiol Actions

PLK1

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

64kda
Polo样激酶1（PLK-1）是一种丝氨酸-苏氨酸蛋白激酶，在细胞周期的M期起着多种重要功能，包括调节中心体成熟和纺锤体组装、去除染色体臂（chromosome arm）的粘连蛋白、APC/C 抑制剂的失活、有丝分裂结束和胞质分裂的调节。 表达的PLK-1 蛋白在G2和M 期聚集至最高水平，在有丝分裂以后和整个G1期下降至几乎不可测的水平，然后在S期再次开始聚集。

Immunogen

对应于人Polo样激酶1（PLK1）的6His标记全长融合蛋白。也称丝氨酸-苏氨酸蛋白激酶PLK。

Other Notes

浓度：请参考批次特异性浓缩物的检验报告。

Physical form

形式：纯化
纯化蛋白A
蛋白A纯化小鼠IgG2b，溶于含有0.07 M Tris-甘氨酸（pH 7.4）、0.105 M NaCl、0.035％叠氮化钠和30％甘油的缓冲液中。 储存于 -20°C。

Preparation Note

自收到之日起可稳定保存1年。

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

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存储类别

10 - Combustible liquids

wgk

WGK 1

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Neuronal c-Abl activation leads to induction of cell cycle and interferon signaling pathways.
Schlatterer, SD; Suh, HS; Conejero-Goldberg, C; Chen, S; Acker, CM; Lee, SC; Davies, P
Journal of Neuroinflammation null
The family of polo-like kinases.
Golsteyn, R M, et al.
Progress in Cell Cycle Research, 2, 107-114 (1996)
Hitesh Patel et al.
Journal of molecular cell biology, 8(4), 338-348 (2016-03-20)
Loss of Kindlin 1 (Kin1) results in the skin blistering disorder Kindler Syndrome (KS), whose symptoms also include skin atrophy and reduced keratinocyte proliferation. Kin1 binds to integrins to modulate their activation and more recently it has been shown to
Chieh-Yuan Cheng et al.
Oncotarget, 9(30), 21231-21243 (2018-05-17)
BI2536 has been developed as a potential therapeutic agent for various cancers but not in oral cancer cells. Since BI2536 exhibits mitosis-regulating activity which are the most radiosensitive, we hypothesized that BI2536 might modulate the radiosensitivity of oral cancer cells.
Zhiguo Li et al.
Cancer research, 77(12), 3169-3180 (2017-05-18)
The mitotic kinase Plk1 contributes to the DNA damage response (DDR) by targeting multiple factors downstream of the core responder kinase ATM/ATR. In this study, we show that Polo-like kinase 1 (Plk1) also phosphorylates key factors upstream of ATM/ATR and
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