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Merck
CN

05-902R

抗-HA标记抗体,克隆114-2C-7,兔单克隆

clone 114-2C-7, Upstate®, from rabbit

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702
Clone:
114-2C-7, monoclonal
Technique(s):
western blot: suitable
Application:
WB
Citations:
10
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biological source

rabbit

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

114-2C-7, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Upstate®

technique(s)

western blot: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

unmodified

General description

人流感血凝素(HA)是人病毒感染性所需的表面糖蛋白。HA标签来源于氨基酸98-106对应的HA分子。它已被广泛用作表达载体中的通用表位标签。已经设计了许多重组蛋白以表达HA标签,该标签似乎不干扰重组蛋白的生物活性或生物分布。该标签有助于蛋白质的检测,分离和纯化。05-902R是兔单克隆目录号05-902的重组版本。

Immunogen

合成肽,含CYPYDVPDY,来源于流感病毒血凝素(HA)蛋白。

Application

抗HA标签抗体,克隆114-2C-7,检测HA标签的水平,已发布,经验证可用于WB。
研究子类别
表位标签
研究类别
表位标签 & 一般用途

Biochem/physiol Actions

该抗体可识别含有HA表位标签的重组蛋白。

Physical form

形式:纯化
纯化蛋白A
蛋白A纯化兔免疫球蛋白,溶于缓冲液,含60mM Tris、90mm NaCl、0.03% NaN3、60mM甘氨酸(pH 7.5)和40%甘油。

Preparation Note

自运送之日起在-20°C可稳定保存1年。等分以避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始小瓶进行离心。

Analysis Note

通过蛋白质印迹HA标记WNT-1转染HEK293细胞进行常规评估。

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。


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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Manuel Osorio-Valeriano et al.
Molecular cell, 81(19), 3992-4007 (2021-09-26)
ParB-like CTPases mediate the segregation of bacterial chromosomes and low-copy number plasmids. They act as DNA-sliding clamps that are loaded at parS motifs in the centromere of target DNA molecules and spread laterally to form large nucleoprotein complexes serving as
Anna Nordin et al.
Genome biology, 24(1), 185-185 (2023-08-11)
Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is an increasingly popular technique to map genome-wide binding profiles of histone modifications, transcription factors, and co-factors. The ENCODE project and others have compiled blacklists for ChIP-seq which have been widely adopted:
Dario Zimmerli et al.
eLife, 9 (2020-08-19)
BCL9 and PYGO are β-catenin cofactors that enhance the transcription of Wnt target genes. They have been proposed as therapeutic targets to diminish Wnt signaling output in intestinal malignancies. Here we find that, in colorectal cancer cells and in developing



全球贸易项目编号

货号GTIN
05-902R04053252281976