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05-904

Sigma-Aldrich

抗HA标签抗体

Upstate®, from mouse

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eCl@ss:
32160702
NACRES:
NA.43

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

monoclonal

species reactivity

vertebrates (common species)

manufacturer/tradename

Upstate®

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同位素/亚型

IgG3

运输

dry ice

target post-translational modification

unmodified

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05-902RSAB430060304-902
Anti-HA Tag Antibody Upstate®, from mouse

Sigma-Aldrich

05-904

抗HA标签抗体

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

culture supernatant

clone

monoclonal

clone

114-2C-7, monoclonal

clone

polyclonal

clone

DW2, monoclonal

species reactivity

vertebrates (common species)

species reactivity

-

species reactivity

-

species reactivity

vertebrates (common species)

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

western blot: suitable

technique(s)

western blot: 1:500-1:1000

technique(s)

western blot: suitable

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一般描述

人流感血凝素(HA)是人病毒感染性所需的表面糖蛋白。HA标签衍生自对应于氨基酸98-106的HA分子,已被广泛用作表达载体中的通用表位标签。已经设计了许多重组蛋白以表达HA标签,该标签似乎不干扰重组蛋白的生物活性或生物分布。该标签有助于蛋白质的检测,分离和纯化。

特异性

与其他物种的反应性尚未得到确认。
识别细胞中过表达的HA标记蛋白,包括转染哺乳动物细胞中靶蛋白的氨基或羧基末端。

免疫原

对应于流感血凝素氨基酸98-106的KLH偶联的合成肽

应用

使用经过验证可用于IC、IP & WB的该抗-HA标签抗体检测HA标签。
免疫沉淀:
独立实验室已报告5 μg/mL his抗体可免疫沉淀重组HA标记的融合蛋白。

免疫细胞化学:
一个独立的实验室已报告5 μg/mL的该抗体可对HA标记的融合蛋白进行免疫染色。
研究子类别
表位标签
研究类别
表位标签 & 一般用途

质量

通过蛋白质印迹法对HEK293细胞RIPA裂解液中氨基末端含有HA标签的重组蛋白进行常规评估。

蛋白质印迹分析:
0.5-2 μg/mL该批次检测到来自HEK293细胞的RIPA裂解液中氨基末端含有HA标签的重组蛋白。

目标描述

各种

联系

替代:07-221

外形

形式:纯化
纯化的小鼠单克隆IgG3,溶于含有PBS(pH 7.4,含有0.05%叠氮化钠)的缓冲液中。
蛋白A纯化

储存及稳定性

自收到之日起在-20°C可稳定保存1年。

处理建议:
第一次融化后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。 分装到微量离心管中,并储存于 -20°C。 避免反复冻融循环,以免损坏 IgG 和影响产品性能。

分析说明

对照
来自HEK293细胞的RIPA裂解液

其他说明

浓度:请参考批次特异性浓缩物的分析证书。

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

10 - Combustible liquids

WGK

WGK 2


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