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UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702
Clone:
monoclonal
Species reactivity:
vertebrates (common species)
Application:
immunocytochemistry
immunoprecipitation (IP)
western blot
immunoprecipitation (IP)
western blot
Technique(s):
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Citations:
101
产品名称
抗HA标签抗体, Upstate®, from mouse
isotype
IgG3
biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
monoclonal
species reactivity
vertebrates (common species)
manufacturer/tradename
Upstate®
technique(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Analysis Note
对照
来自HEK293细胞的RIPA裂解液
来自HEK293细胞的RIPA裂解液
通过蛋白质印迹法对HEK293细胞RIPA裂解液中氨基末端含有HA标签的重组蛋白进行常规评估。
蛋白质印迹分析:
0.5-2 μg/mL该批次检测到来自HEK293细胞的RIPA裂解液中氨基末端含有HA标签的重组蛋白。
蛋白质印迹分析:
0.5-2 μg/mL该批次检测到来自HEK293细胞的RIPA裂解液中氨基末端含有HA标签的重组蛋白。
Application
使用经过验证可用于IC、IP & WB的该抗-HA标签抗体检测HA标签。
免疫沉淀:
独立实验室已报告5 μg/mL his抗体可免疫沉淀重组HA标记的融合蛋白。
免疫细胞化学:
一个独立的实验室已报告5 μg/mL的该抗体可对HA标记的融合蛋白进行免疫染色。
独立实验室已报告5 μg/mL his抗体可免疫沉淀重组HA标记的融合蛋白。
免疫细胞化学:
一个独立的实验室已报告5 μg/mL的该抗体可对HA标记的融合蛋白进行免疫染色。
研究子类别
表位标签
表位标签
研究类别
表位标签 & 一般用途
表位标签 & 一般用途
Biochem/physiol Actions
与其他物种的反应性尚未得到确认。
识别细胞中过表达的HA标记蛋白,包括转染哺乳动物细胞中靶蛋白的氨基或羧基末端。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
人流感血凝素(HA)是人病毒感染性所需的表面糖蛋白。HA标签衍生自对应于氨基酸98-106的HA分子,已被广泛用作表达载体中的通用表位标签。已经设计了许多重组蛋白以表达HA标签,该标签似乎不干扰重组蛋白的生物活性或生物分布。该标签有助于蛋白质的检测,分离和纯化。
各种
Immunogen
对应于流感血凝素氨基酸98-106的KLH偶联的合成肽
Other Notes
替代:07-221
浓度:请参考批次特异性浓缩物的分析证书。
Physical form
形式:纯化
纯化的小鼠单克隆IgG3,溶于含有PBS(pH 7.4,含有0.05%叠氮化钠)的缓冲液中。
蛋白A纯化
Preparation Note
自收到之日起在-20°C可稳定保存1年。
处理建议:
第一次融化后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。 分装到微量离心管中,并储存于 -20°C。 避免反复冻融循环,以免损坏 IgG 和影响产品性能。
处理建议:
第一次融化后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。 分装到微量离心管中,并储存于 -20°C。 避免反复冻融循环,以免损坏 IgG 和影响产品性能。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
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