产品名称
抗-IRS1抗体, Upstate®, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
human, mouse, rat
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... IRS1(3667)
Analysis Note
对照
阳性抗原对照：货号12-305，3T3/A31裂解液。加入2.5 μL 2-巯基乙醇/100 μL裂解液，煮沸5分钟以还原样本。在微凝胶每个泳道上样20 μg经还原的裂解液。
阳性抗原对照：货号12-305，3T3/A31裂解液。加入2.5 μL 2-巯基乙醇/100 μL裂解液，煮沸5分钟以还原样本。在微凝胶每个泳道上样20 μg经还原的裂解液。
通过蛋白质印迹在NIH3T3细胞裂解液中进行了常规评估。
Application
抗IRS1抗体是针对IRS1的抗体，可用于IP & WB。
研究子类别
胰岛素/能量信号传导
胰岛素/能量信号传导
研究类别
信号传导
信号传导
Biochem/physiol Actions
可识别IRS1，Mr 160-185kDa.。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
165 kDa
胰岛素受体底物（IRS）分子是胰岛素信号传导的关键介质，在维持基本的细胞功能（例如生长，存活和代谢）中起着核心作用。它们充当胰岛素受体与包含Src同源2（SH2）域的细胞内信号分子复杂网络之间的对接蛋白。已鉴定出该家族的四个成员（IRS-1，IRS-2，IRS-3，IRS-4）在组织分布，亚细胞定位，发育表达，与胰岛素受体的结合以及与含蛋白质SH2结构域的相互作用方面有所不同。小鼠IRS基因靶向破坏的结果为这些相关分子之间的功能差异提供了重要线索，表明它们在体内起着不同而特定的作用。现有数据与以下观点一致：IRS-1和IRS-2在负责葡萄糖产生（肝脏），葡萄糖摄取（骨骼肌和脂肪组织）和胰岛素产生（胰腺β细胞）的组织中在功能上不可互换。实际上，IRS-1似乎在骨骼肌中起主要作用，而IRS-2似乎调节肝胰岛素的作用以及胰腺β细胞的发育和存活。相比之下，IRS-3和IRS-4基因似乎在IRS信号系统中起着多余的作用。据报道，在肥胖和2型糖尿病等胰岛素抵抗状态下，肌肉IRS-1表达和功能存在缺陷。
Immunogen
大鼠肝脏IRS1的羧基末端14个氨基酸肽（[C]YASINFQKQPEDRQ）
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
免疫亲和纯化
纯化的兔IgG，溶于含有0.2 M Tris-甘氨酸（pH 7.4）、0.15 M NaCl、0.1 mM EDTA、5 mg/mL BSA和0.05%叠氮化钠的缓冲液中。
Preparation Note
自收到之日起在-20°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品，在融化后和取下盖子之前，将原始样品瓶进行离心。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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