07-1016
抗-磷酸化-Upf1（Ser1127）抗体
from rabbit, purified by affinity chromatography
别名:
Regulator of nonsense transcripts 1, ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, NORF1, Up-frameshift suppressor 1 homolog, hUpf1
产品名称
抗-磷酸化-Upf1（Ser1127）抗体, from rabbit, purified by affinity chromatography
biological source
rabbit
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
mouse, human
species reactivity (predicted by homology)
chicken (based on 100% sequence homology), zebrafish (based on 100% sequence homology), yeast (based on 100% sequence homology), bovine (based on 100% sequence homology), rat (based on 100% sequence homology)
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pSer1127)
Quality Level
Gene Information
human ... UPF1(5976)
Analysis Note
已通过蛋白质印迹在Calyculin A和冈田酸处理的NIH3T3中进行了评估。
蛋白质印迹分析：该抗体的1:1000稀释液在用Calyculin A（50 nM）和冈田酸（500 nM）处理30分钟的NIH3T3的10 µg裂解液中检测到磷酸化UPF1（Ser1127）。
蛋白质印迹分析：该抗体的1:1000稀释液在用Calyculin A（50 nM）和冈田酸（500 nM）处理30分钟的NIH3T3的10 µg裂解液中检测到磷酸化UPF1（Ser1127）。
Application
抗磷酸化UPF1（Ser1127）（目录号07-1016）是一种高度特异性的兔多克隆抗体，其靶向在Ser1127处磷酸化的无义转录物1（RENT1）的调节剂，并已在免疫沉淀，肽抑制测定和蛋白质印迹中进行了测试。
蛋白印迹分析: 来自代表性批次的1:1000稀释液在10 µg用Calyculin A和冈田酸处理的A431中检测到磷酸化Upf1 (Ser1127)。
肽抑制分析：来自代表性批次的1:1,000稀释液可阻断Calyculin A和冈田酸处理的NIH3T3中的磷酸化-Upf1（Ser1127）。
免疫沉淀分析：5 µg来自代表性批次的产品在用Calyculin a和冈田酸处理的NIH3T3中免疫沉淀磷酸化-UPF1（Ser1127）。
肽抑制分析：来自代表性批次的1:1,000稀释液可阻断Calyculin A和冈田酸处理的NIH3T3中的磷酸化-Upf1（Ser1127）。
免疫沉淀分析：5 µg来自代表性批次的产品在用Calyculin a和冈田酸处理的NIH3T3中免疫沉淀磷酸化-UPF1（Ser1127）。
Biochem/physiol Actions
该兔多克隆抗体可检测多种物种中无义转录本1（RENT1）的调节剂。它靶向磷酸化Ser1127周围9个氨基酸内的表位。
General description
无义转录本1的调节剂（UniProt：Q92900；又称ATP依赖性解旋酶RENT1，无义mRNA还原因子1，NORF1，上移码抑制因子1同源物，hUpf1）由人UPF1（又称KIAA0221，RENT1）基因（基因ID：5976）编码。Upf1是Dna2/Nam7解旋酶家族的成员。它是含有过早终止密码子的mRNA的无义介导的衰变（NMD）所需的RNA依赖性解旋酶和ATPase。它在翻译终止后被募集到mRNA，并经历磷酸化和去磷酸化的循环。它被SMG1磷酸化，磷酸化被认为是NMD中必不可少的步骤，是形成mRNA监视复合物所必需的。磷酸化的Upf1被Est1b/SMG5，SMG6和SMG7识别，它们提供了与涉及核酸外切和核酸内切途径的mRNA降解机制的联系。超磷酸化形式靶向P体，而未磷酸化的蛋白质分布在整个细胞质中。Upf1的ATPase活性是分解经历NMD的mRNP所必需的，并且对于胚胎生存能力也是必不可少的。据报道，通过选择性剪接产生了Upf1的两种亚型。Upf1包含Upf1型锌指结构域（aa 121-272）和核苷酸结合结构域（aa 506-510）。
观测值〜140 kDa。计算值为125 kDa。
Immunogen
对应于Ser1127磷酸化的Upf1的KLH偶联线性肽。
表位：磷酸化Ser1127
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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