07-1231
抗-Notch1抗体，细胞内
serum, from rabbit
别名:
Neurogenic locus notch homolog protein 1 precursor, Notch (Drosophila) homolog 1 (translocation-associated), Notch 1, Notch homolog 1, translocation-associated (Drosophila), Translocation-associated notch protein TAN-1, Neurogenic locus notch homolog pro
产品名称
抗-Notch1抗体，细胞内, serum, from rabbit
biological source
rabbit
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human
should not react with
mouse
technique(s)
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... NOTCH1(4851)
Analysis Note
已通过蛋白质印迹对Hela裂解液进行了常规评估。
蛋白质印迹分析：该批次的产品已以1：500的稀释度在10 μg HeLa裂解液中检测到NOTCH1，细胞内（裂解形式）。
蛋白质印迹分析：该批次的产品已以1：500的稀释度在10 μg HeLa裂解液中检测到NOTCH1，细胞内（裂解形式）。
Application
ELISA：先前批次的1:20,000-1:100,000稀释液用于针对肽免疫原的标准夹心ELISA测定。
最佳工作稀释液必须由最终用户确定。
免疫组织化学（石蜡）：
先前批次的代表性测试。
具有表位检索的最佳IGF-1染色：脂肪瘤。
最佳工作稀释液必须由最终用户确定。
免疫组织化学（石蜡）：
先前批次的代表性测试。
具有表位检索的最佳IGF-1染色：脂肪瘤。
使用经ELISA、WB、IHC(P)验证的细胞内（兔多克隆抗体）抗Notch1抗体检测Notch1（也称为神经源性基因座notch同源蛋白1前体）。
Biochem/physiol Actions
Notch 1，细胞内。对人Notch 2无反应性。
与其他物种的反应性尚未确定。
General description
Notch是单程跨膜受体蛋白家族，在内质网中以无活性形式合成，并在识别后被反式高尔基体网络中的弗林蛋白酶样转化酶（S1裂解）在细胞外部位点进行蛋白水解裂解。RQRR序列在以异二聚体的形式到达质膜之前产生活性的，配体可及的形式。Notch家族由4个成员（1-4）组成，其配体包括Delta和Jagged配体家族。这些配体引起Notch的蛋白水解以释放细胞内结构域。切割产生C末端片段N(TN)和N末端片段N(EC)。配体结合后，它被TNF-α转化酶（TACE）裂解（S2裂解），产生与膜相关的中间片段，称为Notch细胞外截短（NEXT）。然后，该片段被gly1743和val1744之间的早老素依赖性γ-分泌酶切割（S3切割），以从膜释放细胞内结构域（NICD）。该结构域易位至细胞核并通过结合DNA结合蛋白Csl启动转录事件。Notch家族成员参与细胞的分化和发育。
~80 kDa
Immunogen
来自人Notch 1的合成肽。
Other Notes
替代：04-1046
Physical form
兔多克隆IgG血清溶于含有0.02 M磷酸钾、0.15 M NaCl和0.01%叠氮化钠的缓冲液中。
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Ying Xu et al.
Andrology, 10(8), 1644-1659 (2022-09-05)
In the testis, spermatocytes and spermatids rely on lactate produced by Sertoli cells (SCs) as energy source. Transforming growth factor-beta 3 (TGF-β3) is one of the generally accepted paracrine regulatory factors of SC-created blood-testis barrier (BTB), yet its role in
Elenaé Vázquez-Ulloa et al.
Oncotarget, 9(27), 18916-18928 (2018-05-04)
The participation of NOTCH signaling in invasive cervical cancer (ICC) remains controversial since both tumor suppressive and oncogenic properties have been described. Additionally, the role of NUMB, a negative regulator of NOTCH, remains unclear in ICC. We aimed to investigate
LaNisha L Patterson et al.
Infection and immunity, 91(9), e0000223-e0000223 (2023-08-18)
Ehrlichia chaffeensis has evolved multiple strategies to evade innate defenses of the mononuclear phagocyte. Recently, we reported the E. chaffeensis tandem repeat protein (TRP)120 effector functions as a Notch ligand mimetic and a ubiquitin ligase that degrades the nuclear tumor
Xiaoyi Liu et al.
Gland surgery, 10(11), 3116-3127 (2021-12-21)
Deltex 1 (DTX1) is a single transmembrane protein with ubiquitin E3 ligase activity which has been found to play a role in the development of several cancers. We aimed to investigate the associations between DTX1 and breast cancer (BC). We
LaNisha L Patterson et al.
mBio, 13(2), e0007622-e0007622 (2022-04-01)
Ehrlichia chaffeensis evades innate host defenses by reprogramming the mononuclear phagocyte through mechanisms that involve the exploitation of multiple evolutionarily conserved cellular signaling pathways, including Notch. This immune evasion strategy is directed in part by tandem repeat protein (TRP) effectors.
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