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Merck
CN

07-175

抗磷酸化-JNK (Thr183/Tyr185, Thr221/Tyr223) 抗体

from rabbit, purified by affinity chromatography

别名:

JNK1 alpha protein kinase, JNK1 beta protein kinase, JUN N-terminal kinase, Stress-activated protein kinase JNK1, Stress-activated protein kinase JNK1, mitogen-activated protein kinase 8, mitogen-activated protein kinase 8 isoform JNK1 alpha1, mitogen-ac

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

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产品名称

抗磷酸化-JNK (Thr183/Tyr185, Thr221/Tyr223) 抗体, from rabbit, purified by affinity chromatography

biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human, mouse, horse

species reactivity (predicted by homology)

canine (based on 100% sequence homology), bovine (based on 100% sequence homology), chicken (based on 100% sequence homology), rat (based on 100% sequence homology), Xenopus (based on 100% sequence homology), equine (based on 100% sequence homology)

technique(s)

activity assay: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

NP_620634

UniProt accession no.

P45983

shipped in

wet ice

target post-translational modification

phosphorylation (p(Thr183/pTyr185), (pThr221/pTyr223))

Quality Level

100

Gene Information

human ... MAPK8(5599)

相关类别

Analysis Note

对照
未处理和λ磷酸酶处理的RAW264.7细胞裂解液
通过蛋白质印迹法在未处理和λ磷酸酶处理的RAW264.7细胞裂解液中进行了评估。

蛋白质印迹分析：2 µg/mL的该抗体在10 µg未处理和λ磷酸酶RAW264.7细胞裂解液中检测到JNK。

Application

使用经验证可用于WB，EA & IP的抗磷酸化-JNK（Thr183/Tyr185，Thr221/Tyr223）抗体检测磷酸化-JNK（Thr183/Tyr185）。
免疫沉淀分析：10 µg先前批次对RAW264.7细胞裂解液在Thr183/Tyr185和Thr221/Tyr223磷酸化的JNK进行免疫沉淀。
研究子类别
MAP激酶
研究类别
信号传导

Biochem/physiol Actions

该抗体可识别Thr183/Tyr185和Thr221/Tyr223磷酸化的JNK。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

JNK1（MAPK8）是MAP激酶家族的成员。MAP激酶作为多种生化信号的整合点，参与增殖、分化、转录调控和发育等多种细胞过程。该激酶被各种细胞刺激激活，并靶向特定的转录因子，从而响应细胞刺激而介导立即早期的基因表达。发现肿瘤坏死因子α（TNF-α）激活该激酶是TNF-α诱导的细胞凋亡所必需的。该激酶也参与紫外线辐射诱导的细胞凋亡，被认为与细胞色素c介导的细胞死亡途径有关。对该基因的小鼠对应物的研究表明，该激酶在T细胞增殖，凋亡和分化中起关键作用。已经报道了编码不同亚型的四个可变剪接的转录物变体。JNK1被两个双重特异性激酶MAP2K4和MAP2K7之一的苏氨酸和酪氨酸磷酸化激活。JNK途径与糖尿病密切相关，肥胖症的水平异常升高。细胞可渗透的JNK抑制肽可能有望作为糖尿病的治疗剂。
观测值〜42 kDa。 在40-50 kDa之间有几种亚型

Immunogen

与在Thr183/Tyr185和Thr221/Tyr223处磷酸化的人JNK相对应的KLH偶联线性肽。
表位：在Thr183/Tyr185和Thr221/Tyr223处磷酸化

Other Notes

浓度：请参考批次特异性浓缩物的分析证书。

Physical form

亲和纯化
纯化的兔多克隆抗体，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。

Preparation Note

自收到之日起，在2-8°C条件下可稳定保存1年。

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存储类别

10 - Combustible liquids

wgk

WGK 1

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