07-507
抗磷酸化-BMK1/Erk5 (Thr218/Tyr220) 抗体
Upstate®, from rabbit
别名:
Anti-Anti-BMK1, Anti-Anti-ERK4, Anti-Anti-ERK5, Anti-Anti-PRKM7
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选择尺寸
关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
polyclonal
Species reactivity:
rat, mouse, bovine, human
Application:
inhibition assay
western blot
western blot
Technique(s):
inhibition assay: suitable (peptide)
western blot: suitable
western blot: suitable
Citations:
11
Uniprot accession no.:
产品名称
抗磷酸化-BMK1/Erk5 (Thr218/Tyr220) 抗体, Upstate®, from rabbit
biological source
rabbit
antibody form
affinity purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
purified by
affinity chromatography
species reactivity
rat, mouse, bovine, human
manufacturer/tradename
Upstate®
technique(s)
inhibition assay: suitable (peptide)
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
phosphorylation (pThr218/pTyr220)
Quality Level
Gene Information
bovine ... Mapk7(537703)
human ... MAPK7(5598)
mouse ... Mapk7(23939)
rat ... Mapk7(114509)
Analysis Note
通过蛋白质印迹/肽抑制分析在HeLa细胞裂解液中进行了评估。
蛋白质印迹/肽抑制分析： 该抗体的1：1000稀释液在HeLa细胞裂解液中检测到磷酸化-BMK1/Erk5（Thr218/Tyr220）。
蛋白质印迹/肽抑制分析： 该抗体的1：1000稀释液在HeLa细胞裂解液中检测到磷酸化-BMK1/Erk5（Thr218/Tyr220）。
对照
使用山梨醇处理的HeLa细胞
使用山梨醇处理的HeLa细胞
Application
研究子类别
MAP激酶
MAP激酶
研究类别
信号传导
信号传导
肽抑制分析：
用抗磷酸化BMK 1/ERK5（Thr218/Tyr220）（1µg/mL（1:1000）稀释）（目录号07-507）探测HeLa细胞裂解液，HeLa细胞裂解液+非磷酸化蛋白和HeLa细胞裂解液+磷酸化肽。使用与HRP偶联的驴抗兔IgG二抗和化学发光检测系统观察蛋白质。
箭头表示HeLa细胞裂解液&HeLa细胞裂解液+非磷酸化肽上的BMK1/ERK5（〜115 kDa）。HeLa细胞裂解液+磷酸化肽段上不存在条带，表明该抗体成功抑制了肽段。
用抗磷酸化BMK 1/ERK5（Thr218/Tyr220）（1µg/mL（1:1000）稀释）（目录号07-507）探测HeLa细胞裂解液，HeLa细胞裂解液+非磷酸化蛋白和HeLa细胞裂解液+磷酸化肽。使用与HRP偶联的驴抗兔IgG二抗和化学发光检测系统观察蛋白质。
箭头表示HeLa细胞裂解液&HeLa细胞裂解液+非磷酸化肽上的BMK1/ERK5（〜115 kDa）。HeLa细胞裂解液+磷酸化肽段上不存在条带，表明该抗体成功抑制了肽段。
该抗磷酸化-BMK1/Erk5（Thr218, Tyr220）抗体经过验证可用在WB中检测磷酸化-BMK1/Erk5（Thr218, Tyr220）。
Biochem/physiol Actions
识别磷酸化-BMK1/Erk5（Thr218, Tyr220）。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
110-115kDa
Immunogen
对应于人BMK1/Erk5氨基酸213-224的KLH偶联的合成肽
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
亲和纯化的免疫球蛋白，溶于0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠中。
免疫亲和纯化
Preparation Note
自收到之日起，在2-8°C可稳定保存1年。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
Shan Jiang et al.
BioMed research international, 2017, 6895730-6895730 (2017-05-26)
Background. The aim of this study was to assess the effects of low concentrations of H2O2 on angiogenesis of human umbilical vein endothelial cells (HUVECs) in vitro and explore the underlying mechanisms. Methods. HUVECs were cultured and stimulated with different
Cathleen R Carlin et al.
Molecular biology of the cell, 34(12), ar116-ar116 (2023-08-30)
The polarized distribution of membrane proteins into apical and basolateral domains provides the basis for specialized functions of epithelial tissues. The EGF receptor (EGFR) plays important roles in embryonic development, adult-epithelial tissue homeostasis, and growth and survival of many carcinomas.
Zeynep Madak-Erdogan et al.
Molecular cancer research : MCR, 12(5), 714-727 (2014-02-08)
Cancer cell motility and invasiveness are fundamental characteristics of the malignant phenotype and are regulated through diverse signaling networks involving kinases and transcription factors. This study establishes an estrogen receptor (ERα)/MAPK (ERK5)/cofilin (CFL1) network that specifies the degree of breast
G Zhou et al.
The Journal of biological chemistry, 270(21), 12665-12669 (1995-05-26)
We have identified two components of a new protein kinase signaling cascade, MAPK/ERK kinase 5 (MEK5) and extracellular signal-regulated kinase 5 (ERK5). The MEK5 cDNA was isolated by degenerate PCR and encodes a 444-amino acid protein, which has approximately 40%
Kazuhiro Nakamura et al.
Methods in molecular biology (Clifton, N.J.), 661, 91-106 (2010-09-03)
Extracellular signal-regulated kinase 5 (ERK5) is also known as big MAPK (BMK1) or MAPK7. ERK5 is 115 kDa in mass and therefore larger than the other MAPKs such as ERK1/2, JNK, and p38. Like other MAPKs, ERK5 is ubiquitously expressed
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