07-641
Anti-MAP2K1/MEK1 Antibody
Upstate, rabbit polyclonal
别名:
Anti-CFC3, Anti-MAPKK1, Anti-MEK1, Anti-MEL, Anti-MKK1, Anti-PRKMK1
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关于此项目
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Clone:
polyclonal
Species reactivity:
mouse, rat, human
Application:
immunoprecipitation (IP)
western blot
western blot
Technique(s):
immunoprecipitation (IP): suitable
western blot: suitable
western blot: suitable
Citations:
37
Uniprot accession no.:
产品名称
MEK1抗体, Upstate®, from rabbit
biological source
rabbit
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
manufacturer/tradename
Upstate®
technique(s)
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MAP2K1(5604)
Analysis Note
对照
阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。 添加 2.5µL 2-巯基乙醇/100µL 裂解液，煮沸5分钟以还原制剂。在微凝胶每个泳道上样20µg经还原的裂解液 。
阳性抗原对照：货号12-301，未刺激的A431细胞裂解液。 添加 2.5µL 2-巯基乙醇/100µL 裂解液，煮沸5分钟以还原制剂。在微凝胶每个泳道上样20µg经还原的裂解液 。
通过免疫印迹法对A431和3T3/A31细胞的RIPA裂解液进行了常规评估。
Application
研究子类别
MAP激酶
MAP激酶
研究类别
信号传导
信号传导
这种MEK1抗体经验证可用于IP、WB检测MEK1。
Biochem/physiol Actions
MEK1
根据序列同源性预测会与兔发生交叉反应。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
45kDa
Immunogen
对应于人MEK1氨基酸2-18（PKKKPTPIQLNPAPDGS）的肽。
Other Notes
替代：04-376
Physical form
70%储存缓冲液（0.1M Tris-甘氨酸（pH 7.4）、0.15M NaCl、0.05％叠氮化钠）和30％甘油。
形式：纯化
纯化蛋白A
Preparation Note
在-20°C下可保存2年
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
Nucleotide sequence of the yeast regulatory gene STE7 predicts a protein homologous to protein kinases.
Teague, M A, et al.
Proceedings of the National Academy of Sciences of the USA, 83, 7371-7375 (1986)
Brian J Girard et al.
The Journal of biological chemistry, 292(1), 339-350 (2016-11-25)
Cytoplasmic localization of proline, glutamic acid, leucine-rich protein 1 (PELP1) is observed in ∼40% of women with invasive breast cancer. In mouse models, PELP1 overexpression in the mammary gland leads to premalignant lesions and eventually mammary tumors. In preliminary clinical
cDNA cloning of MAP kinase kinase reveals kinase cascade pathways in yeasts to vertebrates.
Kosako, H, et al.
The Embo Journal, 12, 787-794 (1993)
Jason M Warfel et al.
Toxins, 3(10), 1278-1293 (2011-11-10)
Systemic anthrax disease is characterized by vascular leakage pathologies. We previously reported that anthrax lethal toxin (LT) induces human endothelial barrier dysfunction in a cell death-independent manner with actin stress fiber formation and disruption of adherens junctions (AJs). In the
Felice D'Agnillo et al.
PloS one, 8(4), e62576-e62576 (2013-04-30)
Vascular leakage pathologies such as pleural effusion and hemorrhage are hallmarks of anthrax pathogenesis. We previously reported that anthrax lethal toxin (LT), the major virulence factor of anthrax, reduces barrier function in cultured primary human microvascular endothelial cells. Here, we
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