产品名称
抗组蛋白H3抗体，CT，pan, serum, Upstate®
biological source
rabbit
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
human, mouse, yeast, rat, chicken, Saccharomyces cerevisiae
manufacturer/tradename
Upstate®
technique(s)
ChIP: suitable
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... H3C1(8350)
mouse ... H3C1(360198)
rat ... H3C1(679994)
Analysis Note
通过免疫印迹法对未经处理的丁酸钠或秋水仙胺处理的HeLa细胞的酸提取蛋白进行常规评估。
蛋白质印迹分析：
该批次的1:25,000-1:50,000稀释液在未经处理的丁酸钠或秋水仙胺处理的HeLa细胞的酸提取蛋白中以修饰独立的方式检测到组蛋白H3。
蛋白质印迹分析：
该批次的1:25,000-1:50,000稀释液在未经处理的丁酸钠或秋水仙胺处理的HeLa细胞的酸提取蛋白中以修饰独立的方式检测到组蛋白H3。
Application
使用在ChIP、WB中验证的抗组蛋白H3抗体、CT、pan（兔多克隆抗体）检测组蛋白H3，也称为H3、组蛋白H3、H3组蛋白家族成员T、组蛋白3 H3、组蛋白簇3 H3。
染色质免疫沉淀：
一个独立的实验室已经报道了该抗体可从酵母中免疫沉淀染色质。
一个独立的实验室已经报道了该抗体可从酵母中免疫沉淀染色质。
Biochem/physiol Actions
可识别组蛋白H3的C端区域，Mr 17 kDa
由于序列同源性，预期存在广泛的物种交叉反应性。
General description
组蛋白H3是参与真核细胞染色质结构的五种主要组蛋白之一。H3具有一个主要的球状结构域和一个长N末端的尾巴，其与核糖体核小体′串珠′结构体的结构有关。组蛋白H3的N末端尾部从球状核小体核心突起，其可能发生几种不同类型的表观遗传修饰，从而影响细胞过程。这些修饰包括甲基或乙酰基与赖氨酸和精氨酸的共价连接，以及丝氨酸或苏氨酸的磷酸化。
大约17 kDa
Immunogen
与人组蛋白H3的C末端相对应的KLH偶联的合成肽。
Other Notes
浓度：请参考批次特异性浓缩物的分析证书。
Physical form
兔抗血清在储存缓冲液（0.02 M磷酸盐、0.25 M NaCl、0.1%叠氮化钠）中按1:2稀释，然后加入甘油至 30%。
Legal Information
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 1
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