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Merck
CN

09-403

抗-TKS5 (SH3 #1) 抗体

from rabbit

别名:

Adaptor protein TKS5, Five SH3 domain-containing protein, SH3 and PX domains 2A, SH3 multiple domains 1, SH3 multiple domains protein 1, five SH3 domains

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunocytochemistry
immunoprecipitation (IP)
western blot
Species reactivity:
mouse, human
Citations:
6
Technique(s):
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Uniprot accession no.:
O89032

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产品名称

抗-TKS5 (SH3 #1) 抗体, from rabbit

biological source

rabbit

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

technique(s)

immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

NM_008018.3

UniProt accession no.

O89032

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... SH3PXD2A(9644)
mouse ... Sh3Pxd2A(14218)

相关类别

Analysis Note

对照
蛋白质印迹：
NIH/3T3（100%融合）裂解液
免疫细胞化学：
小鼠3T3-Src(Y527F)细胞
通过蛋白质印迹在NIH/3T3（100%融合）裂解液中进行了常规评估。

Application

抗TKS5 (SH3 #1)抗体可检测TKS5 (SH3 #1) 水平 & 已出版&并经过验证可用于WB，IC & IP。
研究子类别
细胞骨架信号转导
研究类别
细胞结构
蛋白质印迹分析：抗体可在20 μg NIH/3T3（100%融合）裂解液中检测到TKS5。

Biochem/physiol Actions

其他同源性：人、犬、马、大鼠和牛（100%序列同源性），鸡（93%序列同源性）

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

130-150 kDa 某些细胞裂解液中可能会出现未表征的条带。
Tks5（以前称为Fish）是一种大型支架蛋白，具有一个氨基末端PX结构域和五个SH3结构域。它在正常成纤维细胞中是细胞质的。在Src转化的细胞中，Tks5定位于足小体。Tks5和组成型活性Src在上皮癌细胞中的共表达导致足小体/侵袭性伪足的出现。Tks5表达降低的细胞通过基质胶的侵袭性很差。Tks5在一些侵袭性人类癌细胞系和肿瘤组织中表达并定位于足小体/侵袭性伪足，特别是乳腺癌和黑色素瘤。因此，Tks5似乎是足小体/侵袭性伪足形成和某些癌细胞侵袭所必需的。

Immunogen

表位：166-225 aa
重组蛋白，aa：166-225。同源性：人、犬、马、大鼠、牛（100%，60/60）；鸡（93%，56/60）

Other Notes

浓度：请参考批次特异性浓缩物的分析证书。

Physical form

在含0.05％NaN3的0.1M Tris-甘氨酸（pH7.4）150mM NaCl中的纯化抗体。
形式：纯化
蛋白A纯化

Preparation Note

自收到之日起，在2-8°C以未稀释的等分试样可保存1年。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Karla C Williams et al.
Oncogene, 38(19), 3598-3615 (2019-01-18)
Invadopodia are cell protrusions that mediate cancer cell extravasation but the microenvironmental cues and signaling factors that induce invadopodia formation during extravasation remain unclear. Using intravital imaging and loss of function experiments, we determined invadopodia contain receptors involved in chemotaxis
Samuel J Ghilardi et al.
Molecular biology of the cell, 32(18), 1707-1723 (2021-07-01)
Interactions between the actin cytoskeleton and the plasma membrane are important in many eukaryotic cellular processes. During these processes, actin structures deform the cell membrane outward by applying forces parallel to the fiber's major axis (as in migration) or they
Nawal Bendris et al.
Journal of cell science, 129(14), 2804-2816 (2016-06-10)
The ability of cancer cells to degrade the extracellular matrix and invade interstitial tissues contributes to their metastatic potential. We recently showed that overexpression of sorting nexin 9 (SNX9) leads to increased cell invasion and metastasis in animal models, which
Deciphering the involvement of the Hippo pathway co-regulators, YAP/TAZ in invadopodia formation and matrix degradation.
Venghateri, et al.
Cell Death & Disease, 14, 290-290 (2023)
Sara K Donnelly et al.
The Journal of cell biology, 216(12), 4331-4349 (2017-10-25)
The initial step of metastasis is the local invasion of tumor cells into the surrounding tissue. Invadopodia are actin-based protrusions that mediate the matrix degradation necessary for invasion and metastasis of tumor cells. We demonstrate that Rac3 GTPase is critical
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