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Merck
CN

09-864

抗 JHDM1B 抗体

from rabbit, purified by affinity chromatography

别名:

JMJD, lysine (K)-specific demethylase 2B, F-box protein FBL10, [Histone-H3]-lysine-36 demethylase 1B, JEMMA (Jumonji domain, EMSY-interactor, methyltransferase motif) protein, F-box and leucine-rich repeat protein 10, protein containing CXXC domain 2, ju

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
immunocytochemistry
western blot
Species reactivity:
mouse, human, rat
Citations:
30
Technique(s):
immunocytochemistry: suitable
western blot: suitable
Uniprot accession no.:
Q8NHM5

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产品名称

抗 JHDM1B 抗体, from rabbit, purified by affinity chromatography

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, human, rat

species reactivity (predicted by homology)

chimpanzee (based on 100% sequence homology), rabbit (based on 100% sequence homology), porcine (based on 100% sequence homology), horse (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

NP_115979

UniProt accession no.

Q8NHM5

shipped in

wet ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... KDM2B(84678)

相关类别

Analysis Note

对照
HeLa核提取物
通过蛋白质印迹对HeLa核提取物进行了评估。

蛋白质印迹分析：1 µg/mL的该抗体在10 µg HeLa核提取物中检测到JHDM1B。

Application

免疫细胞化学分析：代表性批次的1：500稀释液在NIH/3T3和HeLa细胞中检测到JHDM1B。
免疫细胞化学分析：2 µg/mL代表批次在A431细胞中检测到JHDM1B。
抗JHDM1B抗体是一种兔多克隆抗体，用于检测JHDM1B（也称为赖氨酸（K）特异性脱甲基酶2B，F-box蛋白FBL10，[组蛋白-H3]-赖氨酸-36脱甲基酶1B） & 已在WB，ICC中进行了验证。
研究子类别
核受体
研究类别
表观遗传学&核功能

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

含有JmjC结构域的组蛋白去甲基化蛋白1B，也称为赖氨酸特异性脱甲基酶2B，是由人的JHDM1B基因（也称为KDM2B）编码的核酶。JHDM1A/KDM2B表现出H3K36特异性脱甲基酶活性，相对于H3K36me和H3K36me3（PMID 16362057），更优先选择H3K36me2。据报道，JHDM1A/KDM2B通过靶向rDNA从而以JmjC域依赖性方式抑制rRNA的转录来负调控细胞增殖（PMID 17994099）。
观测值〜165 kDa。在一些裂解液中可以观察到未表征的较低分子量带。

Immunogen

对应于人JHDM1B的KLH偶联线性肽。

Other Notes

浓度：请参考批次特异性浓缩物的分析证书。

Physical form

亲和纯化
纯化的兔多克隆抗体，溶于含有0.1 M Tris-甘氨酸（pH 7.4）、150 mM NaCl和0.05％叠氮化钠的缓冲液中。

Preparation Note

自收到之日起，在2-8°C条件下可稳定保存1年。

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存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

分析证书(COA)

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Manabu Ozawa et al.
Biology of reproduction, 94(4), 92-92 (2016-03-18)
The F-box and leucine-rich repeat protein 10 (Fbxl10) gene encodes a protein that catalyzes demethylation of H3K4 and H3K36. In this study, we show the important roles of FBXL10 as a histone demethylase in sustainable sperm production using mice in
Nefeli Zacharopoulou et al.
Cancer biology & therapy, 21(6), 533-540 (2020-03-17)
Recent studies revealed that the histone demethylase KDM2B regulates the epithelial markers E-Cadherin and ZO-1, the RhoA/B/C-small-GTPases and actin cytoskeleton organization, in DU-145 prostate- and HCT-116 colon-tumor cells. Here we addressed the role of KDM2B in the activation of Focal
Yan Kuang et al.
Cancer science, 112(1), 231-242 (2020-10-23)
MicroRNA let-7b is a potent tumor suppressor and targets crucial oncogenes. Previous studies have shown that let-7b expression is suppressed in ovarian cancer; however, the regulatory mechanisms of let-7b in ovarian cancer are still not well defined. The cellular role
Tsuyoshi Fukuda et al.
Molecular and cellular neurosciences, 46(3), 614-624 (2011-01-12)
Histone methylation is the important transcription regulatory system that affects mammalian development and cell differentiation. Alterations in epigenetic gene regulation are associated with disease. Fbxl10 (F-box and leucine-rich repeat protein 10) is a JmjC domain-containing histone demethylase. Although Fbxl10 has
Polycomb group and SCF ubiquitin ligases are found in a novel BCOR complex that is recruited to BCL6 targets.
Gearhart, MD; Corcoran, CM; Wamstad, JA; Bardwell, VJ
Molecular and cellular biology null
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